BM-40（骨粘连蛋白，SPARC）在成人皮肤的表皮和真皮区室中表达。,Journal of Investigative Dermatology

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BM-40（骨粘连蛋白，SPARC）在成人皮肤的表皮和真皮区室中表达。
Journal of Investigative Dermatology ( IF 5.7 ) Pub Date : 1998-02-11 , DOI: 10.1046/j.1523-1747.1998.00094.x
N Hunzelmann ₁ , M Hafner , S Anders , T Krieg , R Nischt

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BM-40（Osteonectin，SPARC）是人类成骨细胞分泌的最丰富的糖蛋白。对小鼠组织中BM-40 mRNA表达进行的原位杂交研究表明，骨骼中转录本的水平最高，但在其他一些间充质组织中也观察到了表达。相反，关于BM-40在人组织，特别是皮肤中的表达知之甚少。通过RNA印迹分析从正常人皮肤获得的总RNA，并显示出BM-40的明显表达。为了在体内分析其表达，进行了原位杂交，证明了BM-40在真皮的成纤维细胞，平滑肌和内皮细胞中表达。有趣的是，在成人皮肤表皮的整个基底，棘突和颗粒层中也检测到了BM-40 mRNA。免疫组织化学进一步分析显示，真皮中明显沉积，在乳头状真皮基底膜下方，血管以及腺体结构附近沉积最强烈。在表皮中，可以在上基底层的细胞间检测到BM-40蛋白。通过免疫荧光在培养的角质形成细胞中检测到的BM40的细胞间沉积进一步支持了这一发现。这项研究表明，以前被认为仅在细胞外基质产生细胞中表达的BM-40实际上可能在表皮的分化和维持中起作用。在表皮中，可以在上基底层的细胞间检测到BM-40蛋白。通过免疫荧光在培养的角质形成细胞中检测到的BM40的细胞间沉积进一步支持了这一发现。这项研究表明，以前被认为仅在细胞外基质产生细胞中表达的BM-40实际上可能在表皮的分化和维持中起作用。在表皮中，可以在上基底层的细胞间检测到BM-40蛋白。通过免疫荧光在培养的角质形成细胞中检测到的BM40的细胞间沉积进一步支持了这一发现。这项研究表明，以前被认为仅在细胞外基质产生细胞中表达的BM-40实际上可能在表皮的分化和维持中起作用。

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BM-40 (osteonectin, SPARC) is expressed both in the epidermal and in the dermal compartment of adult human skin.

BM-40 (Osteonectin, SPARC) is the most abundant glycoprotein secreted by human osteoblasts. In situ hybridization studies on the expression of BM-40 mRNA in murine tissues have demonstrated the highest levels of transcripts in bone, but expression was also observed in several other mesenchymal tissues. In contrast, little is known about the expression of BM-40 in human tissues, especially in skin. Total RNA obtained from normal human skin was analyzed by northern blotting and revealed a marked expression of BM-40. To analyze its expression in vivo, in situ hybridization was performed, demonstrating that BM-40 is expressed in fibroblasts, smooth muscle, and endothelial cells in the dermis. Interestingly, BM-40 mRNA was also detected throughout the basal, spinous, and granular layers in the epidermis of adult human skin. Further analysis by immunohistochemistry revealed a marked deposition in the dermis that was most intense directly below the basement membrane in the papillary dermis and around vascular as well as glandular structures. In the epidermis, BM-40 protein could be detected intercellularly in suprabasal layers. This finding is further supported by the intercellular deposition of BM40 detected by immunofluorescence in cultured keratinocytes. This study demonstrates that BM-40 that has previously been thought to be exclusively expressed in extracellular matrix producing cells may in fact play a role in differentiation and maintenance of the epidermis.

更新日期：2019-11-01

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