2,6-二氯苄腈破坏纤维素合成会影响拟南芥的细胞骨架结构和细胞壁结构。,Plant Biology

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2,6-二氯苄腈破坏纤维素合成会影响拟南芥的细胞骨架结构和细胞壁结构。
Plant Biology ( IF 4.2 ) Pub Date : 2012-07-05 , DOI: 10.1111/j.1438-8677.2012.00630.x
L Peng ₁ , L Zhang , X Cheng , L-S Fan , H-Q Hao

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纤维素是植物细胞壁的主要成分，并且是工业原料的重要来源。尽管纤维素生物合成是植物生物学中最重要的生化过程之一，但纤维素合成的调控机制仍不清楚。在这里，我们报告2，6-二氯苄腈（DCB），一种纤维素合成抑制剂，以剂量和时间依赖性方式抑制拟南芥根的发育。用DCB处理后，植物细胞壁显示出改变的纤维素分布和强度，如钙荧光白和S4B染色所示。此外，当用针对果胶表位的单克隆抗体JIM5免疫染色时，在DCB存在下果胶沉积减少。使用傅里叶变换红外（FTIR）分析证实了这一结果。共聚焦显微镜显示，在低浓度DCB的存在下，微管细胞骨架的组织被显着破坏，而肌动蛋白细胞骨架仅在高浓度DCB的作用下显示出变化。另外，用N-ST-YFP标记的高尔基体和用VHA-a1-GFP标记的TGN的亚细胞动力学都被DCB部分阻断。透射电子显微镜表明，细胞壁结构受DCB的影响，高尔基体也是如此。扫描电子显微镜显示纤维素微纤维的组织变化。这些结果表明，DCB抑制纤维素合成不仅会引起根细胞壁化学成分和细胞骨架结构的改变，而且还会改变纤维素微纤维的分布，

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Disruption of cellulose synthesis by 2,6-dichlorobenzonitrile affects the structure of the cytoskeleton and cell wall construction in Arabidopsis.

Cellulose is the major component of plant cell walls and is an important source of industrial raw material. Although cellulose biosynthesis is one of the most important biochemical processes in plant biology, the regulatory mechanisms of cellulose synthesis are still unclear. Here, we report that 2,6-dichlorobenzonitrile (DCB), an inhibitor of cellulose synthesis, inhibits Arabidopsis root development in a dose- and time-dependent manner. When treated with DCB, the plant cell wall showed altered cellulose distribution and intensity, as shown by calcofluor white and S4B staining. Moreover, pectin deposition was reduced in the presence of DCB when immunostained with the monoclonal antibody JIM5, which was raised against pectin epitopes. This result was confirmed using Fourier transform infrared (FTIR) analysis. Confocal microscopy revealed that the organisation of the microtubule cytoskeleton was significantly disrupted in the presence of low concentrations of DCB, whereas the actin cytoskeleton only showed changes with the application of high DCB concentrations. In addition, the subcellular dynamics of Golgi bodies labelled with N-ST-YFP and TGN labelled with VHA-a1-GFP were both partially blocked by DCB. Transmission electron microscopy indicated that the cell wall structure was affected by DCB, as were the Golgi bodies. Scanning electron microscopy showed changes in the organisation of cellulose microfibrils. These results suggest that the inhibition of cellulose synthesis by DCB not only induced changes in the chemical composition of the root cell wall and cytoskeleton structure, but also changed the distribution of cellulose microfibrils, implying that cellulose plays an important role in root development in Arabidopsis.

更新日期：2019-11-01

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