OBJECTIVE To investigate the effects of occupational exposure to formaldehyde on the micronuclei frequencies in peripheral blood lymphocytes of workers. METHODS Two hundred thirty six plywood workers were divided into 3 exposure groups (low, middle and high) according to internal exposure biomarker (formaldehyde human serum albumin conjugate, FA-HSA), which was detected by ELISA. The concentrations of formaldehyde (FA) in air of two workshops were measure using the high performance liquid chromatography. Cytokinesis-block micronucleus (CBMN) test was used to detect the micronuclei frequencies of peripheral blood lymphocyte in 236 workers. RESULTS The average concentrations of FA in the low and high exposure workshops were 0.58 +/- 0.20 and 1.48 +/- 0.61 mg/m3, respectively, there was significant difference (P < 0.01). The average concentrations of serum FA-HAS of workers in two workshops were 69.22 +/- 15.37 and 136.29 +/- 89.49 pg/ml, respectively, there was significant difference (P < 0.01). The results of CBMN test showed that the micronucleus frequencies in low, middle and high exposure groups were 1.94 +/- 1.72, 2.10 +/- 1.92 and 2.10 +/- 1.70 per thousand, respectively, there were no significant differences between groups. However, the micronucleus frequencies in accumulative low, middle and high exposure groups were 1.36 +/- 1.36, 2.31 +/- 1.81 and 2.49 +/- 1.92 per thousand, respectively, there were significant differences between different accumulative exposure groups (P < 0.01). The results of correlation analysis indicated that there was a positive correlation between accumulative exposure levels and micronucleus frequencies (r(s) = 0.321, P < 0.01). The accumulative exposure doses may be a risk factor for high micronucleus frequencies in workers exposed to FA (P(trend) = 0.002). CONCLUSION FA-HSA levels can serve as an internal exposure biomarker for assessing the exposure level of workers exposed to FA. Accumulative formaldehyde exposure resulted in an increase of micronuclei frequencies of peripheral blood lymphocyte in plywood workers.

中文翻译：

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胶合板工人累积暴露于甲醛中外周血淋巴细胞微核频率的增加

目的探讨职业性接触甲醛对工人外周血淋巴细胞微核频率的影响。方法将236名胶合板工人根据内部暴露生物标志物（甲醛人血清白蛋白结合物FA-HSA）分为3个暴露组（低，中，高），通过ELISA法对其进行检测。使用高效液相色谱法测量了两个车间空气中的甲醛（FA）浓度。采用胞质分裂阻滞微核（CBMN）试验检测236名工人外周血淋巴细胞的微核频率。结果低暴露车间和高暴露车间中FA的平均浓度分别为0.58 +/- 0.20和1.48 +/- 0.61 mg / m3，差异有统计学意义（P <0.01）。两个车间工人的血清FA-HAS的平均浓度分别为69.22 +/- 15.37 pg / ml和136.29 +/- 89.49 pg / ml，差异有统计学意义（P <0.01）。CBMN测试结果显示，低，中和高暴露组的微核频率分别为每千个1.94 +/- 1.72、2.10 +/- 1.92和2.10 +/- 1.70，两组之间无显着差异。然而，累积的低，中和高暴露组的微核频率分别为每千个1.36 +/- 1.36、2.31 +/- 1.81和2.49 +/- 1.92，不同的累积暴露组之间存在显着差异（P <0.01 ）。相关分析结果表明，累积暴露水平与微核频率之间存在正相关（r（s）= 0.321，P <0.01）。累积暴露剂量可能是暴露于FA的工人中高微核频率的危险因素（P（趋势）= 0.002）。结论FA-HSA水平可以作为内部暴露生物标志物，用于评估暴露于FA的工人的暴露水平。甲醛的累积暴露导致胶合板工人外周血淋巴细胞的微核频率增加。