脂多糖（LPS）对人脐血管内皮细胞（HUVECs）的形态和功能的影响,Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns

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脂多糖（LPS）对人脐血管内皮细胞（HUVECs）的形态和功能的影响
Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns Pub Date : 2002-03-06
J Cheng ₁ , J Yuan , J Zheng

Affiliation

目的研究脂多糖（LPS）对HUVECs的形态和功能的影响，以探讨脂多糖（LPS）活化的血管内皮细胞（VEC）在全身炎症反应，败血症和MOF中的作用。方法采用体外培养的HUVECs为模型。倒置显微镜观察LPS对HUVECs形态的影响。ELISA用于评估IL-6含量。用共聚焦激光扫描荧光显微镜通过免疫荧光染色法测定ICAM-1的表达。结果LPS可改变HUVEC的形态，使其呈长绳状。刺激HUVEC分泌IL-6的LPS的最小剂量为1 ng / ml，与LPS浓度的增加平行地增强了作用，并在烧伤后8小时达到最高水平。激光共聚焦扫描显微镜观察，LPS（10 ng / ml）培养24小时后，HUVECs的核酸和细胞质膜上ICAM-1的表达明显增加。结论LPS可明显影响HUVEC的形态和功能。结果，LPS可能在增加血管通透性，促进白细胞粘附和引发炎症级联反应中起重要作用。

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[The effect of Lipopolysacharide (LPS) on morphology and function of human umbilical endothelial cells (HUVECs)].

OBJECTIVE To study the effects of LPS on the morphology and function of HUVECs, so as to explore the roles of activated vascular endothelial cells (VEC) by LPS in systemic inflammatory reaction, sepsis and MOF. METHODS HUVECs primarily cultured in vitro were employed as the model. Inverted microscope was used to observe the effects of LPS on the morphology of HUVECs. ELISA was used to assess the IL-6 content. The expression of ICAM-1 was determined by immunofluorescent staining method with confocal laser-scanning fluorescence microscope. RESULTS The morphology of HUVECs could be altered by LPS to become long cord-like in shape. The smallest dose of LPS for stimulating the secretion of IL-6 from HUVEC was 1 ng/ml, and the effect was enhanced parallel to the increase of the concentration of LPS and reached top level at 8 postburn hours. The expresson of ICAM-1 on the nucleic and cytoplasmic membrane of HUVECs increased obviously after HUVECs was cultured with LPS (10 ng/ml) for 24 hours when observed by laser confocal scanning light microscope. CONCLUSION The morphology and function of HUVECs could be evidently affected by LPS. As a result, LPS might play important roles in the increase of vascular permeability, the promotion of leukocytic adherence, and the initiation of inflammatory cascade reaction.

更新日期：2019-11-01

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