MACROCALYX 和 JOINTLESS 在番茄果实脱落区发育的转录调控中相互作用。,Plant Physiology

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MACROCALYX 和 JOINTLESS 在番茄果实脱落区发育的转录调控中相互作用。
Plant Physiology ( IF 6.5 ) Pub Date : 2011-11-21 , DOI: 10.1104/pp.111.183731
Toshitsugu Nakano ₁ , Junji Kimbara , Masaki Fujisawa , Mamiko Kitagawa , Nao Ihashi , Hideo Maeda , Takafumi Kasumi , Yasuhiro Ito

Affiliation

植物的脱落是一个重要的过程，用于在衰老、受损或成熟时脱落器官，如叶、花和果实。脱离发生在称为脱离区 (AZ) 的预定位置。虽然果实脱落的调控对农业至关重要，但其发育机制仍不清楚。在这里，我们描述了一种调节番茄 (Solanum lycopersicum) 花梗 AZ 发育的新型转录因子。我们发现番茄花梗 AZ 的发育需要 MACROCALYX (MC) 基因，该基因以前被鉴定为萼片大小调节器并编码 MADS-box 转录因子。MC与拟南芥(Arabidopsis thaliana) FRUITFULL具有显着的序列相似性，后者参与调控果实开裂区的发育。MC 蛋白与另一种 MADS-box 蛋白 JOINTLESS 发生物理相互作用，JOINTLESS 被称为果实脱落调节剂；由此产生的异源二聚体获得了特定的 DNA 结合活性。在脱落前阶段对花梗的转录组分析表明，涉及植物激素相关功能、细胞壁修饰、脂肪酸代谢和转录因子的基因的表达受 MC 和 JOINTLESS 调节。受调控的基因包括拟南芥 WUSCHEL 的同源基因、腋分生组织调节因子、杯状子叶和横向抑制因子。这些拟南芥基因编码表征良好的转录因子，调节植物组织中的分生组织维持、腋生分生组织发育和边界形成。番茄同源物在 AZ 中特异性表达，但在其他花梗组织中不表达，

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MACROCALYX and JOINTLESS interact in the transcriptional regulation of tomato fruit abscission zone development.

Abscission in plants is a crucial process used to shed organs such as leaves, flowers, and fruits when they are senescent, damaged, or mature. Abscission occurs at predetermined positions called abscission zones (AZs). Although the regulation of fruit abscission is essential for agriculture, the developmental mechanisms remain unclear. Here, we describe a novel transcription factor regulating the development of tomato (Solanum lycopersicum) pedicel AZs. We found that the development of tomato pedicel AZs requires the gene MACROCALYX (MC), which was previously identified as a sepal size regulator and encodes a MADS-box transcription factor. MC has significant sequence similarity to Arabidopsis (Arabidopsis thaliana) FRUITFULL, which is involved in the regulation of fruit dehiscent zone development. The MC protein interacted physically with another MADS-box protein, JOINTLESS, which is known as a regulator of fruit abscission; the resulting heterodimer acquired a specific DNA-binding activity. Transcriptome analyses of pedicels at the preabscission stage revealed that the expression of the genes involved in phytohormone-related functions, cell wall modifications, fatty acid metabolism, and transcription factors is regulated by MC and JOINTLESS. The regulated genes include homologs of Arabidopsis WUSCHEL, REGULATOR OF AXILLARY MERISTEMS, CUP-SHAPED COTYLEDON, and LATERAL SUPPRESSOR. These Arabidopsis genes encode well-characterized transcription factors regulating meristem maintenance, axillary meristem development, and boundary formation in plant tissues. The tomato homologs were specifically expressed in AZs but not in other pedicel tissues, suggesting that these transcription factors may play key roles in pedicel AZ development.

更新日期：2019-11-01

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