Using half-leaf method O,O'-diisopropyl (3-(L-1-(benzylamino)-1-oxo-3- phenylpropan-2-yl)thioureido)(phenyl)methyl phosphonate (2009104) was studied for its activity on tobacco mosaic virus (TMV). It showed good curative activity in vivo and the curative activity at 500 μg/mL was found to be 53.3%. In vivo treatment with the control agent Ningnanmycin at 500 μg/mL resulted in 51.2% inhibition and curative inhibition rates respectively. Dot-ELISA test was employed to verify the efficacy of activity of compound 200910 for anti-TMV activity. The mechanism of action of compound 2009104 to resist TMV was also studied. The results showed that the resistance enzymes PAL, POD, SOD activity and chlorophyll content after TMV inoculation K(326) (Nicotiana tabacum K(326)) of tobacco plants followed by treatment with compound 2009104 were significantly enhanced. The study of the effect of compound 2009104 on TMV capsid protein (CP) showed that it inhibited the polymerization process of TMV-CP in vitro.

中文翻译：

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新型含手性膦酸硫脲对烟草花叶病毒的抗病毒活性及作用机制。

使用半叶法研究 O,O'-二异丙基 (3-(L-1-(苄基氨基)-1-氧代-3-苯基丙-2-基)硫脲基)(苯基)甲基膦酸酯 (2009104) 的活性烟草花叶病毒（TMV）。它在体内表现出良好的治疗活性，在500 μg/mL时的治疗活性为53.3%。用 500 μg/mL 的对照剂宁南霉素进行体内治疗，分别产生 51.2% 的抑制率和治愈性抑制率。使用点ELISA测试来验证化合物200910的活性对抗TMV活性的功效。还研究了化合物2009104抗TMV的作用机制。结果表明，抗性酶 PAL、POD、烟草植物 TMV 接种 K(326) (Nicotiana tabacum K(326)) 后 SOD 活性和叶绿素含量显着提高，然后用化合物 2009104 处理。化合物2009104对TMV衣壳蛋白(CP)影响的研究表明，它在体外抑制了TMV-CP的聚合过程。