胰岛素样肽调节果蝇的摄食偏好和代谢。,Frontiers in Physiology

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胰岛素样肽调节果蝇的摄食偏好和代谢。
Frontiers in Physiology ( IF 3.2 ) Pub Date : 2018-09-11 , DOI: 10.3389/fphys.2018.01083
Uliana V Semaniuk ₁ , Dmytro V Gospodaryov ₁ , Khrystyna M Feden'ko ₁ , Ihor S Yurkevych ₁ , Alexander M Vaiserman ₂ , Kenneth B Storey ₃ , Stephen J Simpson ₄ , Oleh Lushchak ₁

Affiliation

果蝇具有八种已鉴定的果蝇胰岛素样肽（DILP），它们参与血淋巴中碳水化合物浓度的调节以及储存代谢产物的积累。在本研究中，我们调查了由dilp1-5和dilp7基因编码的DILP的饮食依赖性作用在调节昆虫食欲，食物选择，甘油三酸酯，糖原，葡萄糖和海藻糖在果蝇体内和碳水化合物中的积累。血淋巴我们已经发现，野生型和突变系显示出碳水化合物的补偿性喂养。但是，在高酵母饮食中，dilp2,3，dilp3，dilp5和dilp7上的突变体显示蛋白质消耗更高。为了评估不同饮食研究品系之间的代谢差异，我们应用了响应面方法。高营养饮食导致野生型果蝇血淋巴中的葡萄糖浓度适度增加。dilp基因的突变改变了这种模式。我们发现，dilp2突变会导致饮食中糖原水平的下降，而dilp3的缺乏会导致循环海藻糖和糖原水平的急剧增加，尤其是在蛋白质消耗量较低的情况下。在所有饮食中，缺乏dilp5会导致糖原和甘油三酸酯水平降低，而敲除dilp7会导致低蛋白消耗时糖原水平增加，同时甘油三酸酯水平下降。果蝇的食欲受到dilp3和dilp7基因的影响。我们的数据有助于了解果蝇作为代谢疾病进一步研究的模型，并可能为揭示代谢调节途径的演变提供指导。

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Insulin-Like Peptides Regulate Feeding Preference and Metabolism in Drosophila.

Fruit flies have eight identified Drosophila insulin-like peptides (DILPs) that are involved in the regulation of carbohydrate concentrations in hemolymph as well as in accumulation of storage metabolites. In the present study, we investigated diet-dependent roles of DILPs encoded by the genes dilp1-5, and dilp7 in the regulation of insect appetite, food choice, accumulation of triglycerides, glycogen, glucose, and trehalose in fruit fly bodies and carbohydrates in hemolymph. We have found that the wild type and the mutant lines demonstrate compensatory feeding for carbohydrates. However, mutants on dilp2,3, dilp3, dilp5, and dilp7 showed higher consumption of proteins on high yeast diets. To evaluate metabolic differences between studied lines on different diets we applied response surface methodology. High nutrient diets led to a moderate increase in concentration of glucose in hemolymph of the wild type flies. Mutations on dilp genes changed this pattern. We have revealed that the dilp2 mutation led to a drop in glycogen levels independently on diet, lack of dilp3 led to dramatic increase in circulating trehalose and glycogen levels, especially at low protein consumption. Lack of dilp5 led to decreased levels of glycogen and triglycerides on all diets, whereas knockout on dilp7 caused increase in glycogen levels and simultaneous decrease in triglyceride levels at low protein consumption. Fruit fly appetite was influenced by dilp3 and dilp7 genes. Our data contribute to the understanding of Drosophila as a model for further studies of metabolic diseases and may serve as a guide for uncovering the evolution of metabolic regulatory pathways.

更新日期：2019-11-01

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