The main objective of this study was to investigate the effects of different doses of spermine and its extended supplementation on the morphology, digestive enzyme activities, and intestinal antioxidant capacity in weaning rats. Nineteen-day-old male rats received intragastric spermine at doses of 0.2 and 0.4 μmol/g BW for 3 or 7 d, whereas control rats received similar doses of saline. The results are as follows: 1) In the jejunum, the seven-day supplementation with both doses of spermine significantly increased crypt depth (P < 0.05) compared with the control group; the supplementation extension of the high spermine dose increased villus height and crypt depth (P < 0.05); in the ileum, the low spermine dose significantly increased villus height and crypt depth compared with the control group for 7 days (P < 0.05). 2) The 3-day supplementation with high spermine dose increased alkaline phosphatase activity in the jejunum (P < 0.05). 3) In the jejunum, the anti-hydroxyl radical (AHR), total superoxide dismutase (T-SOD), catalase (CAT), and total antioxidant capacity (T-AOC) activities were increased (P < 0.05); however, the malondialdehyde (MDA) content was reduced (P < 0.05) in groups supplemented with the high spermine dose relative to those in the control groups after 3 and 7 d; moreover, the anti-superoxide anion (ASA) and glutathione (GSH) contents increased with the high spermine dose that lasted for 3 days (P < 0.05). Furthermore, the T-SOD and CAT activities (after 3 and 7 d), ASA (after 3 d), and AHR (after 7 d) increased with the high spermine dose compared with those of the low spermine dose (P < 0.05). Extending the supplementation duration (7 d) of the high spermine dose decreased the MDA content and ASA and T-AOC activities (P < 0.05). These results suggested that spermine supplementation can modulate gut development and enhance the antioxidant status of the jejunum in weaning rats, and a dosage of 0.4 μmol spermine/g BW had better effects than the dosage of 0.2 μmol spermine/g BW on accelerating gut development and increasing antioxidant capacity.

中文翻译：

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补充精胺对断奶大鼠肠道形态，消化酶活性和肠道抗氧化能力的影响。

这项研究的主要目的是研究不同剂量的精胺及其补充剂对断奶大鼠的形态，消化酶活性和肠道抗氧化能力的影响。十九天大的雄性大鼠在3或7 d内接受0.2和0.4μmol/ g BW剂量的胃内精胺，而对照大鼠则接受相似剂量的生理盐水。结果如下：1）在空肠中，与对照组相比，两种剂量的精胺补充7天显着增加了隐窝深度（P <0.05）；高精胺剂量的补充扩展增加了绒毛高度和隐窝深度（P <0.05）；在回肠中，低精胺剂量比对照组连续7天显着增加了绒毛高度和隐窝深度（P <0.05）。2）高精胺剂量3天补充可提高空肠中碱性磷酸酶的活性（P <0.05）。3）空肠中的抗羟基自由基（AHR），总超氧化物歧化酶（T-SOD），过氧化氢酶（CAT）和总抗氧化能力（T-AOC）活性增加（P <0.05）；然而，在第3和7天后，与对照组相比，补充高精胺剂量的组丙二醛（MDA）含量降低了（P <0.05）。此外，高精胺剂量持续3天后，抗超氧阴离子（ASA）和谷胱甘肽（GSH）含量增加（P <0.05）。此外，高精胺剂量与低精胺剂量相比，T-SOD和CAT活性（3和7 d后），ASA（3 d之后）和AHR（7 d后）增加（P <0.05） 。延长高精胺剂量的补充时间（7 d）会降低MDA含量以及ASA和T-AOC活性（P <0.05）。这些结果表明，添加精胺可以调节断奶大鼠肠道的发育并增强空肠的抗氧化状态，0.4μmol精胺/ g BW的剂量比0.2μmol精胺/ g BW的剂量能更快地促进肠道的发育和生长。提高抗氧化能力。