Obesity is a serious health issue in many industrialized countries. It is a medical condition with excessive levels of fat accumulated in adipocytes. The objective of the present study was to determine the inhibitory effect of 3-chloro-4,5-dihydroxybenzaldehyde (CDB) on adipogenesis in 3T3-L1 adipocyte cells. CDB suppressed the differentiation and decreased lipid accumulation and triglycerides contents in 3T3-L1 adipocytes. Its suppression effect on fat accumulation was mediated via expression of adipogenesis factors (C/EBPα, SREBP-1c, PPARγ, and adiponectin) during adipocyte differentiation in white adipocyte cells. CDB's ability to suppress fat accumulation was increased in a concentration-dependent manner. It inhibited fatty acid synthesis related proteins including FAS, FABP4, leptin, and perilipin. It also increased expression of phosphorylated AMPK in adipocytes cells. These observations suggest that CDB has potential anti-obesity effect with ability to improve metabolic diseases.

中文翻译：

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3-Chloro-4,5-dihydroxybenzaldehyde通过调节脂肪形成转录因子的表达和AMPK激活来抑制3T3-L1脂肪细胞中的脂肪形成。

在许多工业化国家，肥胖是一个严重的健康问题。这是一种医学状况，脂肪细胞中积累了过多的脂肪。本研究的目的是确定3-氯-4,5-二羟基苯甲醛（CDB）对3T3-L1脂肪细胞中脂肪形成的抑制作用。CDB抑制了3T3-L1脂肪细胞的分化并降低了脂质积累和甘油三酸酯含量。它对脂肪积累的抑制作用是通过在白色脂肪细胞分化过程中脂肪生成因子（C /EBPα，SREBP-1c，PPARγ和脂联素）的表达介导的。CDB抑制脂肪积累的能力以浓度依赖性方式增加。它抑制与脂肪酸合成有关的蛋白质，包括FAS，FABP4，瘦素和周脂素。它还增加了脂肪细胞中磷酸化AMPK的表达。这些观察结果表明，CDB具有潜在的抗肥胖作用，具有改善代谢疾病的能力。