Neomangiferin, a natural C-glucosyl xanthone, has recently received a great deal of attention due to its multiple biological activities. In this study, a rapid and sensitive ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC–MS/MS) method for the quantification of neomangiferin in rat plasma was developed. Using chloramphenicol as an internal standard (IS), plasma samples were subjected to a direct protein precipitation process using methanol (containing 0.05% formic acid). Quantification was performed by multiple reactions monitoring (MRM) method, with the transitions of the parent ions to the product ions of m/z 583.1→330.9 for NG and m/z 321.1→151.9 for IS. The assay was shown to be linear over the range of 0.2–400 ng/mL, with a lower limit of quantification of 0.2 ng/mL. Mean recovery of neomangiferin in plasma was in the range of 97.76%–101.94%. Relative standard deviations (RSDs) of intra-day and inter-day precision were both <10%. The accuracy of the method ranged from 94.20% to 108.72%. This method was successfully applied to pharmacokinetic study of neomangiferin after intravenous (2 mg/kg) and intragastric (10 mg/kg) administration for the first time. The oral absolute bioavailability of neomangiferin was estimated to be 0.53%±0.08% with an elimination half-life (t_1/2) value of 2.74±0.92 h, indicating its poor absorption and/or strong metabolism in vivo.

Neomangiferin，一种天然的C-葡萄糖基yl吨酮，由于其多种生物活性，最近受到了广泛的关注。在这项研究中，开发了一种快速灵敏的超高效液相色谱串联质谱（UHPLC–MS / MS）方法定量大鼠血浆中的新芒果苷。使用氯霉素作为内标（IS），血浆样品使用甲醇（含0.05％甲酸）进行直接蛋白沉淀过程。通过多反应监测（MRM）方法进行定量，其中NG的母离子转变为m / z 583.1→330.9，IS的m / z 321.1→151.9。该分析显示在0.2–400 ng / mL范围内呈线性，定量下限为0.2 ng / mL。血浆新芒果苷的平均回收率在97.76％–101.94％的范围内。日内和日间精度的相对标准偏差（RSD）均<10％。该方法的准确度为94.20％至108.72％。首次静脉注射（2 mg / kg）和胃内注射（10 mg / kg）后，该方法成功应用于新芒果苷的药代动力学研究。新芒果苷的口服绝对生物利用度估计为0.53％±0.08％，消除半衰期（t 该方法成功应用于首次静脉注射（2 mg / kg）和胃内注射（10 mg / kg）的新芒果苷的药代动力学研究。新芒果苷的口服绝对生物利用度估计为0.53％±0.08％，消除半衰期（t 该方法成功应用于首次静脉注射（2 mg / kg）和胃内注射（10 mg / kg）的新芒果苷的药代动力学研究。新芒果苷的口服绝对生物利用度估计为0.53％±0.08％，消除半衰期（t_1/2）值为2.74±0.92 h，表明其在体内的吸收不良和/或新陈代谢旺盛。