Functional sugars have attracted attention because of their wide application prospects in the food, cosmetics, and pharmaceutical industries in recent decades. Compared with complex chemical synthesis, enzymatic methods of creating functional sugars, characterized by high specificity, moderate reaction conditions, and sustainability, are favored. D-lyxose isomerase (D-LI, EC 5.3.1.15), an important aldose-ketose isomerase, catalyzes the reverse isomerization reaction between D-xylulose and D-lyxose, as well as D-fructose and D-mannose. D-LI has drawn researchers' attention due to its broad substrate specificity and high potential for enzymatic production of some functional sugars such as D-xylulose, D-mannose, and D-ribose. In this article, an overview of recent advances in the biochemical properties of various D-LIs is explored in detail. Structural analysis, active site identification, and catalytic mechanisms are also provided. Additionally, the applications of D-LIs for functional sugar production, including D-lyxose, D-mannose, and L-ribose, are reviewed in detail in this paper.

中文翻译：

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D-糖异构酶及其在功能性糖生产中的应用。

近年来，功能性糖由于在食品，化妆品和制药行业的广泛应用前景而备受关注。与复杂的化学合成相比，以功能性糖为特征的酶法方法具有很高的特异性，适度的反应条件和可持续性。一种重要的醛糖-酮糖异构酶D-Lyoseose异构酶（D-LI，EC 5.3.1.15）催化D-木酮糖和D-lyxose以及D-果糖和D-甘露糖之间的反向异构化反应。D-LI由于其广泛的底物特异性和酶促生产某些功能性糖（例如D-木酮糖，D-甘露糖和D-核糖）的潜力而引起了研究人员的注意。在本文中，将详细探讨各种D-LI的生化特性方面的最新进展。还提供结构分析，活性位点识别和催化机理。此外，本文详细综述了D-LIs在功能性糖生产中的应用，包括D-lyxose，D-甘露糖和L-核糖。