Ethyl linoleate is an unsaturated fatty acid used in many cosmetics for its various attributes, such as antibacterial and anti-inflammatory properties and clinically proven to be an effective anti-acne agent. In this study, we investigated the effect of ethyl linoleate on the melanogenesis and the mechanism underlying its action on melanogenesis in B16F10 murine melanoma cells. Our results revealed that ethyl linoleate significantly inhibited melanin content and intracellular tyrosinase activity in α-MSH-induced B16F10 cells, but it did not directly inhibit activity of mushroom tyrosinase. Ethyl linoleate inhibited the expression of microphthalmia-associated transcription factor (MITF), tyrosinase, and tyrosinase related protein 1 (TRP1) in governing melanin pigment synthesis. We observed that ethyl linoleate inhibited phosphorylation of Akt and glycogen synthase kinase 3β (GSK3β) and reduced the level of β-catenin, suggesting that ethyl linoleate inhibits melanogenesis through Akt/GSK3β/β-catenin signal pathway. Therefore, we propose that ethyl linoleate may be useful as a safe whitening agent in cosmetic and a potential therapeutic agent for reducing skin hyperpigmentation in clinics.

中文翻译：

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亚油酸乙酯通过Akt /GSK3β/β-catenin信号通路抑制α-MSH诱导的黑色素生成。

亚油酸乙酯是多种化妆品中使用的不饱和脂肪酸，因为它具有多种特性，例如具有抗菌和消炎作用，并在临床上被证明是有效的抗痤疮剂。在这项研究中，我们调查了亚油酸乙酯对B16F10小鼠黑色素瘤细胞中黑色素生成的作用及其对黑色素生成的作用机理。我们的结果表明，亚油酸乙酯显着抑制α-MSH诱导的B16F10细胞中的黑色素含量和细胞内酪氨酸酶活性，但并未直接抑制蘑菇酪氨酸酶的活性。亚油酸乙酯在支配黑色素的合成过程中抑制了小眼科相关转录因子（MITF），酪氨酸酶和酪氨酸酶相关蛋白1（TRP1）的表达。我们观察到亚油酸乙酯抑制Akt和糖原合酶激酶3β（GSK3β）的磷酸化并降低β-catenin的水平，表明亚油酸乙酯通过Akt /GSK3β/β-catenin信号途径抑制黑素生成。因此，我们建议亚油酸乙酯可以用作化妆品中的安全美白剂，以及在临床上减少皮肤色素沉着的潜在治疗剂。