Background. The disinfectants polyhexamethylene biguanide (PHMB) and 1-bromo-3-chloro-5,5-dimethylimidazolidine-2,4-dione (BCDMH) each have limitations. So far, their combined usage has not been examined. In this study, the fungicidal activity of combined disinfectant using PHMB and BCDMH, named PB, against Candida albicans was evaluated. Methods. Suspension quantitative fungicidal test and viable fungi count were used to test fungicidal effects against C. albicans. Coupon corrosion testing was used to evaluate disinfectants’ corrosive effects on stainless steel, copper, and aluminum. The mouse lymphoma assay was used to detect mutations induced by PB. Results and Discussion. Fungicidal activity of the combination of 40 mg/L PHMB and 40 mg/L BCDMH was comparable to, or even better than, those of 600 mg/L PHMB or 640 mg/L BCDMH alone. The combination of 400 mg/L PHMB and 400 mg/L BCDMH exhibited good fungicidal effects in field applications. The combination of 100 mg/L PHMB and 100 mg/L BCDMH did not have corrosive effects on stainless steel and no mutagenic effect was observed under the test conditions. Conclusions. The combination of PHMB and BCDMH has strong fungicidal effects and little metal corrosive and mutagenic effect and can be used as one suitable fungicide for wide household and industrial applications, including shipping containers.

中文翻译：

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聚六亚甲基双胍与 1-溴-3-氯-5,5-二甲基咪唑烷-2,4-二酮组合的杀菌、腐蚀和突变作用。


背景。消毒剂聚六亚甲基双胍 (PHMB) 和 1-溴-3-氯-5,5-二甲基咪唑烷-2,4-二酮 (BCDMH) 各自都有局限性。到目前为止，它们的组合使用尚未得到检验。本研究评估了 PHMB 和 BCDMH 复合消毒剂（PB）对白色念珠菌的杀菌活性。方法。采用悬浮液定量杀菌试验和活菌计数法检测对白色念珠菌的杀菌效果。采用挂片腐蚀测试来评估消毒剂对不锈钢、铜和铝的腐蚀作用。小鼠淋巴瘤试验用于检测 PB 诱导的突变。结果和讨论。 40 mg/L PHMB 和 40 mg/L BCMH 组合的杀菌活性与单独使用 600 mg/L PHMB 或 640 mg/L BCMH 相当，甚至更好。 400 mg/L PHMB 与 400 mg/L BCMH 组合在现场应用中表现出良好的杀菌效果。 100 mg/L PHMB 与 100 mg/L BCMH 组合对不锈钢没有腐蚀作用，在测试条件下未观察到致突变作用。结论。 PHMB和BCDMH的组合具有很强的杀菌作用，并且对金属的腐蚀和诱变作用很小，可以作为一种适合广泛家庭和工业应用（包括海运集装箱）的杀菌剂。