间充质干细胞分泌的VEGF通过旁分泌机制介导内皮祖细胞向内皮细胞的分化。,Molecular Medicine Reports

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间充质干细胞分泌的VEGF通过旁分泌机制介导内皮祖细胞向内皮细胞的分化。
Molecular Medicine Reports ( IF 3.4 ) Pub Date : 2017-11-16 , DOI: 10.3892/mmr.2017.8059
Quanhu Ge ₁ , Hongwei Zhang ₁ , Jixue Hou ₁ , Longfei Wan ₁ , Wenzhe Cheng ₁ , Xiaoyi Wang ₁ , Dan Dong ₁ , Congzhe Chen ₁ , Jie Xia ₁ , Jun Guo ₁ , Xueling Chen ₂ , Xiangwei Wu ₁

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干细胞疗法是缺血性疾病的有前途的治疗策略。间充质干细胞（MSC）和内皮祖细胞（EPC）在骨髓腔和体外培养物中相互粘附。先前我们已经证明，MSC与EPC之间的粘附对于MSC自我更新及其向成骨细胞和软骨细胞的多分化至关重要。在本研究中，研究了EPC和MSC之间的间接通讯对EPC内皮分化潜能的影响，并探讨了MSC介导的EPC分化的分子机制。通过共同培养MSC和EPC，研究了MSCs分泌的血管内皮生长因子（VEGF）通过旁分泌机制对EPC分化的影响。逆转录-定量聚合酶链反应和蛋白质印迹分析用于检测目的基因和蛋白质的表达。本结果表明，将EPC与MSC共培养可增强分化簇31和von Willebrand因子的表达，它们是内皮表型的特异性标志物，因此表明MSC可能在体外影响EPC的内皮分化。VEGF似乎对该过程至关重要。这些发现对于理解MSC和EPC之间的生物学相互作用以及开发基于干细胞的疗法在治疗缺血性疾病中的应用非常重要。本结果表明，将EPC与MSC共培养可增强分化簇31和von Willebrand因子的表达，它们是内皮表型的特异性标志物，因此表明MSC可能在体外影响EPC的内皮分化。VEGF似乎对该过程至关重要。这些发现对于理解MSC和EPC之间的生物学相互作用以及开发基于干细胞的疗法在治疗缺血性疾病中的应用非常重要。本结果表明，将EPC与MSC共培养可增强分化簇31和von Willebrand因子的表达，它们是内皮表型的特异性标志物，因此表明MSC可能在体外影响EPC的内皮分化。VEGF似乎对该过程至关重要。这些发现对于理解MSC和EPC之间的生物学相互作用以及开发基于干细胞的疗法在治疗缺血性疾病中的应用非常重要。

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VEGF secreted by mesenchymal stem cells mediates the differentiation of endothelial progenitor cells into endothelial cells via paracrine mechanisms.

Stem cell therapy is a promising treatment strategy for ischemic diseases. Mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) adhere to each other in the bone marrow cavity and in in vitro cultures. We have previously demonstrated that the adhesion between MSCs and EPCs is critical for MSC self‑renewal and their multi‑differentiation into osteoblasts and chondrocytes. In the present study, the influence of the indirect communication between EPCs and MSCs on the endothelial differentiation potential of EPCs was investigated, and the molecular mechanisms underlying MSC‑mediated EPC differentiation were explored. The effects of vascular endothelial growth factor (VEGF), which is secreted by MSCs, on EPC differentiation via paracrine mechanisms were examined via co‑culturing MSCs and EPCs. Reverse transcription-quantitative polymerase chain reaction and western blot analysis were used to detect the expression of genes and proteins of interest. The present results demonstrated that co‑culturing EPCs with MSCs enhanced the expression of cluster of differentiation 31 and von Willebrand factor, which are specific markers of an endothelial phenotype, thus indicating that MSCs may influence the endothelial differentiation of EPCs in vitro. VEGF appeared to be critical to this process. These findings are important for the understanding of the biological interactions between MSCs and EPCs, and for the development of applications of stem cell‑based therapy in the treatment of ischemic diseases.

更新日期：2019-11-01

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