长期补充肌酸一水合物后，AGAT和GAMT缺陷小鼠的生化和行为表型。,Metabolic Brain Disease

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长期补充肌酸一水合物后，AGAT和GAMT缺陷小鼠的生化和行为表型。
Metabolic Brain Disease ( IF 3.2 ) Pub Date : 2017-08-16 , DOI: 10.1007/s11011-017-0092-3
Furhan Iqbal _{1,

2} , Herald Hoeger ₃ , Gurt Lubec ₁ , Olaf Bodamer ₄

Affiliation

肌酸/磷酸肌酸系统对于细胞磷酸耦合的能量存储和生产至关重要。我们调查了肌酸一水合物补充在两个不同的肌酸缺乏基因敲除小鼠模型中的效用。断奶后，根据女性的精氨酸：甘氨酸酰胺基转移酶（AGAT）和胍基乙酸：甲基转移酶（GAMT）的基因敲除小鼠和野生型小鼠，根据其基因型和膳食补充剂（无肌酸或2％肌酸一水合物补充膳食）进行饮食研究，使用一系列方法行为测试和生化分析。在补充了2％肌酸的AGAT（p = 0.02）和GAMT敲除小鼠（p <0.001）中都观察到Rota棒的性能得到改善。在莫里斯水迷宫探针试验期间，补充肌酸的AGAT基因敲除小鼠花更少的时间到达虚拟平台（p = 0。03），并且比不含肌酸饮食的小鼠更频繁地越过该区域（p = 0.001）。GAMT基因敲除小鼠记录了类似的观察结果。补充肌酸后，AGAT（p = 0.001）和GAMT（p = 0.05）剔除小鼠的尿肌酐浓度增加。补充肌酸有可能改善AGAT和GAMT基因敲除小鼠的神经肌肉协调和空间学习。长期补充肌酸会导致尿肌酐浓度升高，表明基因敲除小鼠的肌酸代谢改善。补充肌酸有可能改善AGAT和GAMT基因敲除小鼠的神经肌肉协调和空间学习。长期补充肌酸会导致尿肌酐浓度升高，表明基因敲除小鼠的肌酸代谢改善。补充肌酸有可能改善AGAT和GAMT基因敲除小鼠的神经肌肉协调和空间学习。长期补充肌酸会导致尿肌酐浓度升高，表明基因敲除小鼠的肌酸代谢改善。

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Biochemical and behavioral phenotype of AGAT and GAMT deficient mice following long-term Creatine monohydrate supplementation.

The creatine/phosphocreatine system is essential for cellular phosphate coupled energy storage and production. We investigated the utility of creatine monohydrate supplementation in two different creatine deficient knockout mouse models. Following weaning, female Arginine: Glycine Amidinotransferase (AGAT) and Guanidinoacetate: methyltransferase (GAMT) knockouts and wild type mice were studied based on their genotypes and dietary supplementation (creatine free or 2% creatine monohydrate supplemented diet) for 10 weeks, using a series of behavioral tests and biochemical analyzes. An improved Rota rod performance was observed in both AGAT (p = 0.02) and GAMT knockout mice (p < 0.001) supplemented with 2% creatine. During Morris water maze probe trial, creatine supplemented AGAT knockout mice took less time to reach virtual platform (p = 0.03) and more frequently crossed this area (p = 0.001) than mice on creatine free diet. Similar observations were recorded for GAMT knockout mice. Urinary creatinine concentrations for AGAT (p = 0.001) and GAMT (p = 0.05) knockout mice were increased following creatine supplementation. Creatine supplementation has a potential to improve neuro-muscular coordination, spatial learning in both AGAT and GAMT knockout mice. Long term Creatine supplementation results in increased urine creatinine concentrations indicating improved creatine metabolism in knockout mice.

更新日期：2019-11-01

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