甘氨酸和亮氨酸二肽的口服给药可改善 UVB 照射的无毛小鼠的皮肤水合作用和弹性,Biomolecules & Therapeutics

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甘氨酸和亮氨酸二肽的口服给药可改善 UVB 照射的无毛小鼠的皮肤水合作用和弹性
Biomolecules & Therapeutics ( IF 3.0 ) Pub Date : 2017-09-01 , DOI: 10.4062/biomolther.2017.089
Jong Il Park ₁ , Ji Eun Lee ₁ , Hyun Jun Shin ₁ , Soonran Song ₂ , Won Kyung Lee ₂ , Jae Sung Hwang ₁

Affiliation

胎盘是一种特殊的器官，含有多种营养物质，如生长因子、矿物质和生物活性肽。甘氨酸和亮氨酸的二肽是猪胎盘提取物 (PPE) 的主要成分，已被用作人类胎盘提取物的替代品。在这项研究中，我们调查了 PPE 的主要肽、甘氨酰-L-亮氨酸 (Gly-Leu)、L-亮氨酰-甘氨酸 (Leu-Gly) 和 L-亮氨酰-L-亮氨酸 (Leu-Leu) 是否会影响皮肤体外和体内的水合作用和弹性。我们发现 Gly-Leu 和 Leu-Gly 二肽诱导正常人表皮角质形成细胞 (NHEK) 中转谷氨酰胺酶 1 的表达，而 Leu-Leu 二肽则不然。用 Gly-Leu 或 Leu-Gly 处理显着增加了 NHEK 中的乙酰透明质酸 (HA) 合成，并且证实了乙酰透明质酸合酶 2 (HAS2) mRNA 水平的上调。此外，在用 Gly-Leu 或 Leu-Gly 二肽处理的 NHEK 中，弹性蛋白酶活性受到抑制。口服 Gly-Leu 或 Leu-Gly 二肽可增加 UVB 照射的无毛小鼠的皮肤水合作用和弹性。响应于口服施用 Gly-Leu 或 Leu-Gly，观察到 UVB 照射的无毛小鼠中 HA 的显着上调。这些结果表明，猪胎盘的主要二肽 Gly-Leu 和 Leu-Gly 是皮肤保湿配方的潜在活性成分。

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Oral Administration of Glycine and Leucine Dipeptides Improves Skin Hydration and Elasticity in UVB-Irradiated Hairless Mice

Placenta is a special organ that contains many nutrients such as growth factors, minerals, and bioactive peptides. Dipeptides of glycine and leucine are major components of porcine placenta extracts (PPE) that has been used as an alternative of human placenta extracts. In this study, we investigated whether major peptides of PPE, Glycyl-L-Leucine (Gly-Leu), L-Leucyl-Glycine (Leu-Gly), and L-Leucyl-L-Leucine (Leu-Leu), affect skin hydration and elasticity in vitro and in vivo. We found that Gly-Leu and Leu-Gly dipeptides induced the expression of transglutaminase 1 in normal human epidermal keratinocytes (NHEKs) whereas Leu-Leu dipeptides did not. Treatment with Gly-Leu or Leu-Gly significantly increased hyaluronan (HA) synthesis in NHEKs and the upregulation of hyaluronan synthase 2 (HAS2) mRNA level was confirmed. In addition, elastase activity was inhibited in NHEKs treated with Gly-Leu or Leu-Gly dipeptides. Oral administration of Gly-Leu or Leu-Gly dipeptides increased skin hydration and elasticity in UVB-irradiated hairless mice. The significant upregulation of HA in UVB-irradiated hairless mice was observed in response to oral administration of Gly-Leu or Leu-Gly. These results suggest that the major dipeptides of porcine placenta, Gly-Leu and Leu-Gly, are potentially active ingredients for skin moisturization formulations.

更新日期：2017-09-01

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