This study aims to investigate the biodegradation of 4,5-dichloro-2-(n-octyl)-3[2H]-isothiazolone (DCOIT) by a brown-rot fungus Gloeophyllum trabeum as well as the involved mechanism. In the present study, the retentions of DCOIT in treated Masson pine (Pinus massoniana) (MP) chips were determined periodically after incubation with G. trabeum. Then a Fenton-like reaction, known as the chelator-mediated Fenton (CMF) chemistry was used to degrade DCOIT that mimics the degradation pathway of DCOIT by typical brown-rot fungi, and the degradation intermediates were further analyzed. The results demonstrated that DCOIT was rapidly depleted in the early stages of incubation by G. trabeum. The CMF treatment was shown to oxidatively decompose DCOIT by producing reactive oxygen species. This evidence suggests that the CMF chemistry employed by brown-rot fungi contributes to the rapid depletion of DCOIT during G. trabeum exposure, although this does not rule out other possible mechanisms for the biodegradation of DCOIT. The new findings give new insights into the mechanism for the biodegradation of organic biocides, and potentially provide an efficient approach for the removal of organic pollutants in the contaminated water.

中文翻译：

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褐腐真菌球根霉降解4,5-二氯-2-（正辛基）-3 [2H]-异噻唑酮（DCOIT）的潜在机理。

这项研究旨在调查褐腐真菌球叶菊对4,5-二氯-2-（正辛基）-3 [2H]-异噻唑酮（DCOIT）的生物降解作用及其机制。在本研究中，在与小梁短孢菌孵育后，定期确定DCOIT在处理过的马尾松（Pinus massoniana）（MP）芯片中的保留量。然后，使用一种类似于Fenton的反应（称为螯合剂介导的Fenton（CMF）化学）来降解DCOIT，该DCOIT模拟典型的褐腐真菌的DCOIT降解途径，并进一步分析了降解中间体。结果表明，DCOIT在培养早期被小白曲霉迅速耗尽。结果表明，CMF处理可通过产生活性氧来氧化分解DCOIT。该证据表明褐腐真菌使用的CMF化学物质有助于在暴露小球菌的过程中DCOIT的快速消耗，尽管这并不排除DCOIT生物降解的其他可能机制。新发现为有机杀生物剂的生物降解机理提供了新见解，并有可能提供一种去除受污染水中有机污染物的有效方法。