Scutellaria barbata D. Don (SB) is a well known formula in traditional Chinese medicine, which exhibits potent anticancer effects on various cancers. Many miRNAs play crucial roles in the regulation of cancer, for instance, miR‑34a functions as a tumor suppressor, and is often downregulated during cancer. In this study, we investigated the role of ECSB in suppressing the growth of human colon cancer HCT‑8 cells, and whether this is mediated by regulation of miR‑34a and its downstream target genes, using real-time PCR and western blot analysis. ECSB treatment significantly inhibited the proliferation of HCT‑8 cells and promoted apoptosis in a dose-dependent manner. In addition, ECSB treatment significantly increased the level of miR‑34a expression and decreased the levels of Bcl-2, Notch1/2 and Jagged1 expression. Furthermore, knockdown of miR‑34a expression through transfection of anti-miR‑34a oligonucleotide was significantly reversed by ECSB treatment. Likewise, knockdown of miR‑34a resulted in significant upregulation of Bcl-2, Notch1/2 and Jagged1 expression, which was reversed following ECSB treatment. Therefore, this study reveals that ECSB inhibited cancer cell growth via promoting apoptosis and inhibiting proliferation, through regulation of miR‑34a. These findings further support the use of ECSB as an effective therapeutic agent against colon cancer.

中文翻译：

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半枝莲D. Don的氯仿级分通过激活miR‑34a抑制结直肠癌细胞的生长。

半枝莲（Scutellaria barbata D. Don，SB）是传统中药中众所周知的配方，对多种癌症均表现出有效的抗癌作用。许多miRNA在癌症的调节中起着至关重要的作用，例如，miR‑34a起到抑癌作用，并且在癌症期间常常被下调。在这项研究中，我们使用实时荧光定量PCR和Western印迹分析研究了ECSB在抑制人结肠癌HCT-8细胞生长中的作用，以及这是否由miR-34a及其下游靶基因的调节介导。ECSB处理以剂量依赖性方式显着抑制HCT-8细胞的增殖并促进凋亡。此外，ECSB处理可显着提高miR‑34a表达水平，并降低Bcl-2，Notch1 / 2和Jagged1表达水平。此外，ECSB处理显着逆转了通过转染抗miR-34a寡核苷酸来抑制miR-34a表达的现象。同样，敲低miR‑34a会导致Bcl-2，Notch1 / 2和Jagged1表达的显着上调，在ECSB处理后这种表达会逆转。因此，这项研究表明ECSB通过调节miR-34a来促进细胞凋亡和抑制增殖，从而抑制了癌细胞的生长。这些发现进一步支持ECSB作为抗结肠癌的有效治疗剂的用途。这项研究表明ECSB通过调节miR‑34a来促进细胞凋亡和抑制增殖，从而抑制了癌细胞的生长。这些发现进一步支持ECSB作为抗结肠癌的有效治疗剂的用途。这项研究表明ECSB通过调节miR-34a来促进细胞凋亡和抑制增殖，从而抑制了癌细胞的生长。这些发现进一步支持了ECSB作为抗结肠癌的有效治疗剂的用途。