Farrerol is a 2,3-dihydro-flavonoid isolated from rhododendron. In this study, a sensitive and selective ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for the determination of farrerol in rat plasma. Liquid-liquid extraction by ethyl ether was used for sample preparation. Chromatographic separation was achieved on an Agilent UHPLC XDB-C18 column (2.1 × 100 mm, 1.8 μm) with water and methanol (30:70, v/v) as the mobile phase. An electrospray source was applied and operated in negative ion mode; selection reaction monitoring was used for quantification using target fragment ions m/z 299 → 179 for farrerol and m/z 267 → 252 for internal standard. Calibration plots were linear in the range of 2.88-1440 ng/mL for farrerol in rat plasma. Intra- and inter-day precisions were <11.6%, and the accuracy ranged from -13.9 to 11.9%. The UHPLC-MS/MS method was successfully applied in pharmacokinetics and bioavailability studies of farrerol in rats.

中文翻译：

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开发和验证用于定量分析大鼠血浆中法瑞罗的灵敏UHPLC-MS / MS方法：在药代动力学和生物利用度研究中的应用。

Farrerol是从杜鹃花中分离得到的2,3-二氢类黄酮。在这项研究中，开发了一种灵敏且选择性的超高效液相色谱-串联质谱（UHPLC-MS / MS）方法，用于测定大鼠血浆中的法罗洛尔。通过乙醚液-液萃取用于样品制备。在Agilent UHPLC XDB-C18色谱柱（2.1×100 mm，1.8μm）上以水和甲醇（30:70，v / v）为流动相进行色谱分离。施加电喷雾源并以负离子模式操作；选择反应监测用于定量，使用Farrerol的目标片段离子m / z 299→179和内标物的m / z 267→252。大鼠血浆中法瑞罗的校准图在2.88-1440 ng / mL范围内呈线性关系。日内和日间精度均低于11.6％，准确度范围为-13.9至11.9％。UHPLC-MS / MS方法已成功应用于法呢罗在大鼠体内的药代动力学和生物利用度研究。