Dalbergioidin（DAL）通过激活PI3K / AKT / SMAD1途径保护MC3T3-E1成骨细胞免受H2O2诱导的细胞损伤。,Naunyn-Schmiedeberg's Archives of Pharmacology

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Dalbergioidin（DAL）通过激活PI3K / AKT / SMAD1途径保护MC3T3-E1成骨细胞免受H2O2诱导的细胞损伤。
Naunyn-Schmiedeberg's Archives of Pharmacology ( IF 3.1 ) Pub Date : 2017-04-05 , DOI: 10.1007/s00210-017-1365-4
Yu-Qin Jin _{1,

2} , Jia-Ling Li ₁ , Jin-Dong Chen ₁ , Chang-Liang Xu ₃ , Huang Li ₁

Affiliation

活性氧（ROS）是骨质疏松症发展中的关键致病因素。Dalbergioidin（DAL）可以从Uraria crinite（一种用作儿童骨骼发育不良的天然食物）中食用的草本植物中分离出来。尽管尚不清楚DAL具有抗骨质疏松作用，但最近的研究表明DAL具有抗骨质疏松作用。我们使用过氧化氢（H2O2）在成骨细胞MC3T3-E1细胞中诱导的有效氧化应激模型，研究DAL对骨质疏松症的保护作用及其潜在的分子机制。结果表明，DAL处理可维持氧化还原平衡，减少MC3T3-E1细胞凋亡，改善碱性磷酸酶活性并提高Runx2，Osterix和BMP2的成骨相关蛋白表达，以抵抗H2O2引起的氧化损伤。DAL对H2O2诱导的MC3T3-E1细胞死亡的保护作用的潜在分子机制可能在于PI3K / AKT / SMAD1细胞信号通路的激活。两者合计，结果表明DAL对骨质疏松症的潜在保护作用与氧化损伤的减少有关，表明DAL可能在骨代谢疾病，特别是骨质疏松症中有用。

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Dalbergioidin (DAL) protects MC3T3-E1 osteoblastic cells against H2O2-induced cell damage through activation of the PI3K/AKT/SMAD1 pathway.

Reactive oxygen species (ROS) is a pivotal pathogenic factor in the development of osteoporosis. Dalbergioidin (DAL) can be isolated from Uraria crinite, an edible herb used as a natural food for childhood skeletal dysplasia. Recent research has implicated DAL as having an antiosteoporosis effect, although the mechanism of this is unclear. We used an effective oxidative stress model, induced by hydrogen peroxide (H2O2) in osteoblastic MC3T3-E1 cells, to investigate the protective effects of DAL in osteoporosis and the underlying molecular mechanisms. The results indicated that treatment with DAL maintained redox balance, reduced MC3T3-E1 cell apoptosis, improved alkaline phosphatase activity, and elevated the osteogenic-related protein expression of Runx2, Osterix, and BMP2 against oxidative damage induced by H2O2. The potential molecular mechanism involved in the protective effect of DAL against H2O2-induced cell death in MC3T3-E1 cells may lie in the activation of the PI3K/AKT/SMAD1 cell signal pathway. Taken together, the results indicated that the potential protective effects of DAL against osteoporosis were linked to a reduction in oxidative damage, suggesting that DAL may be useful in bone metabolism diseases, particularly osteoporosis.

更新日期：2019-11-01

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