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Tetradecanol reduces EL-4 T cell growth by the down regulation of NF-κB mediated IL-2 secretion.
European Journal of Pharmacology ( IF 4.2 ) Pub Date : 2017-02-09 , DOI: 10.1016/j.ejphar.2017.02.002 Jung Up Park 1 , Bok Yun Kang 1 , Hwa-Jeong Lee 2 , Sunoh Kim 3 , Donghyuck Bae 3 , Jong-Hwan Park 4 , Young Ran Kim 1
European Journal of Pharmacology ( IF 4.2 ) Pub Date : 2017-02-09 , DOI: 10.1016/j.ejphar.2017.02.002 Jung Up Park 1 , Bok Yun Kang 1 , Hwa-Jeong Lee 2 , Sunoh Kim 3 , Donghyuck Bae 3 , Jong-Hwan Park 4 , Young Ran Kim 1
Affiliation
Tetradecanol is a straight-chain saturated fatty alcohol purified from Dendropanax morbifera leaves. We found that tetradecanol (30μM) reduced specifically the growth of T cells such as EL-4 T cell and isolated murine CD4+ T cells. In this study, we investigated the effects of tetradecanol on the regulation of interlukin-2 (IL-2), a potent T cell growth factor. Tetradecanol significantly inhibited IL-2 secretion in EL-4 T cells activated with phorbol 12-myristate 13-acetate (PMA) plus ionomycin (Io) and also in isolated murine CD4+ T cells activated with anti-CD3 and anti-CD28 antibodies. Next, we examined the effect of tetradecanol on the transcriptional activity related to IL-2 production in T cells. Tetradecanol decreased PMA/Io-induced promoter activity of NF-κB in EL-4 T cells, but did not show any significant effects on the promoters of activator protein 1 (AP-1) and nuclear factor of activated T cells (NF-AT). Tetradecanol inhibited IκBα degradation and nuclear translocation of NF-κB subunit, p65 in PMA/Io-activated EL-4 T cells. These results suggest that tetradecanol might have immunosuppressive effects on T cell mediated disorders. Using a chronic allergic contact dermatitis model induced by repeated application of oxazolone, we showed that tetradecanol reduced ear thickness induced by oxazolone.
中文翻译:
十四碳四烯酚通过下调NF-κB介导的IL-2分泌而降低EL-4 T细胞的生长。
十四碳四烯酚是从Dendropanax morbifera叶子中提纯的直链饱和脂肪醇。我们发现十四烷醇(30μM)会特别减少T细胞的生长,例如EL-4 T细胞和分离的鼠CD4 + T细胞。在这项研究中,我们调查了十四烷醇对白细胞介素2(IL-2)(一种有效的T细胞生长因子)的调节作用。十四烷基酚显着抑制被佛波醇12-肉豆蔻酸酯13-乙酸酯(PMA)+离子霉素(Io)激活的EL-4 T细胞以及被抗CD3和抗CD28抗体激活的分离的鼠CD4 + T细胞中的IL-2分泌。接下来,我们检查了十四烷醇对与T细胞中IL-2产生有关的转录活性的影响。十四烷酚降低PMA / Io诱导EL-4 T细胞中NF-κB的启动子活性,但对活化蛋白1(AP-1)的启动子和活化T细胞的核因子(NF-AT)没有显示任何显着影响。十八碳四烯酚抑制PMA / Io激活的EL-4 T细胞中IκBα降解和NF-κB亚基p65的核易位。这些结果表明十四烷醇可能对T细胞介导的疾病具有免疫抑制作用。使用由反复应用恶唑酮诱导的慢性过敏性接触性皮炎模型,我们显示十四烷醇可降低由恶唑酮诱导的耳厚。
更新日期:2019-11-01
中文翻译:
![](https://scdn.x-mol.com/jcss/images/paperTranslation.png)
十四碳四烯酚通过下调NF-κB介导的IL-2分泌而降低EL-4 T细胞的生长。
十四碳四烯酚是从Dendropanax morbifera叶子中提纯的直链饱和脂肪醇。我们发现十四烷醇(30μM)会特别减少T细胞的生长,例如EL-4 T细胞和分离的鼠CD4 + T细胞。在这项研究中,我们调查了十四烷醇对白细胞介素2(IL-2)(一种有效的T细胞生长因子)的调节作用。十四烷基酚显着抑制被佛波醇12-肉豆蔻酸酯13-乙酸酯(PMA)+离子霉素(Io)激活的EL-4 T细胞以及被抗CD3和抗CD28抗体激活的分离的鼠CD4 + T细胞中的IL-2分泌。接下来,我们检查了十四烷醇对与T细胞中IL-2产生有关的转录活性的影响。十四烷酚降低PMA / Io诱导EL-4 T细胞中NF-κB的启动子活性,但对活化蛋白1(AP-1)的启动子和活化T细胞的核因子(NF-AT)没有显示任何显着影响。十八碳四烯酚抑制PMA / Io激活的EL-4 T细胞中IκBα降解和NF-κB亚基p65的核易位。这些结果表明十四烷醇可能对T细胞介导的疾病具有免疫抑制作用。使用由反复应用恶唑酮诱导的慢性过敏性接触性皮炎模型,我们显示十四烷醇可降低由恶唑酮诱导的耳厚。