Tetradecanol is a straight-chain saturated fatty alcohol purified from Dendropanax morbifera leaves. We found that tetradecanol (30μM) reduced specifically the growth of T cells such as EL-4 T cell and isolated murine CD4+ T cells. In this study, we investigated the effects of tetradecanol on the regulation of interlukin-2 (IL-2), a potent T cell growth factor. Tetradecanol significantly inhibited IL-2 secretion in EL-4 T cells activated with phorbol 12-myristate 13-acetate (PMA) plus ionomycin (Io) and also in isolated murine CD4+ T cells activated with anti-CD3 and anti-CD28 antibodies. Next, we examined the effect of tetradecanol on the transcriptional activity related to IL-2 production in T cells. Tetradecanol decreased PMA/Io-induced promoter activity of NF-κB in EL-4 T cells, but did not show any significant effects on the promoters of activator protein 1 (AP-1) and nuclear factor of activated T cells (NF-AT). Tetradecanol inhibited IκBα degradation and nuclear translocation of NF-κB subunit, p65 in PMA/Io-activated EL-4 T cells. These results suggest that tetradecanol might have immunosuppressive effects on T cell mediated disorders. Using a chronic allergic contact dermatitis model induced by repeated application of oxazolone, we showed that tetradecanol reduced ear thickness induced by oxazolone.

中文翻译：

---

十四碳四烯酚通过下调NF-κB介导的IL-2分泌而降低EL-4 T细胞的生长。

十四碳四烯酚是从Dendropanax morbifera叶子中提纯的直链饱和脂肪醇。我们发现十四烷醇（30μM）会特别减少T细胞的生长，例如EL-4 T细胞和分离的鼠CD4 + T细胞。在这项研究中，我们调查了十四烷醇对白细胞介素2（IL-2）（一种有效的T细胞生长因子）的调节作用。十四烷基酚显着抑制被佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）+离子霉素（Io）激活的EL-4 T细胞以及被抗CD3和抗CD28抗体激活的分离的鼠CD4 + T细胞中的IL-2分泌。接下来，我们检查了十四烷醇对与T细胞中IL-2产生有关的转录活性的影响。十四烷酚降低PMA / Io诱导EL-4 T细胞中NF-κB的启动子活性，但对活化蛋白1（AP-1）的启动子和活化T细胞的核因子（NF-AT）没有显示任何显着影响。十八碳四烯酚抑制PMA / Io激活的EL-4 T细胞中IκBα降解和NF-κB亚基p65的核易位。这些结果表明十四烷醇可能对T细胞介导的疾病具有免疫抑制作用。使用由反复应用恶唑酮诱导的慢性过敏性接触性皮炎模型，我们显示十四烷醇可降低由恶唑酮诱导的耳厚。