1. Crotonoside is a bioactive ingredient from Croton Herba with a strong antitumour activity. This study aimed to develop a highly sensitive and selective high-performance liquid chromatography (HPLC) method to quantify crotonoside in biological samples for pharmacokinetics and distribution studies.

2. Protein precipitation by perchloric acid was used to separate crotonoside from the biological samples, and the recovery rates for crotonoside and the internal standard (luteoloside) were >80%. All calibration curves examining the crotonoside levels in plasma and tissues were linear (all correlation coefficients > 0.99).

3. The response to crotonoside appeared to be dose disproportional to the maximum plasma concentration and the area under the time–concentration curve in plasma over the range of 12.5–50.0 mg/kg, and crotonoside was highly distributed in tissues after intravenous administration. The highest crotonoside level was detected in the liver (28.79 ± 14.96 μg/g), whereas crotonoside was undetected in the brain.

1.巴豆甙是一种来自巴豆草的生物活性成分，具有很强的抗肿瘤活性。这项研究旨在开发一种高灵敏度和选择性的高效液相色谱（HPLC）方法，以定量生物样品中的巴豆苷，用于药代动力学和分布研究。

2.用高氯酸沉淀的蛋白质从生物样品中分离出巴豆苷，巴豆苷和内标物（黄体苷）的回收率> 80％。所有检查血浆和组织中巴豆苷水平的校准曲线均为线性（所有相关系数> 0.99）。

3.对巴豆苷的反应似乎与最大血浆浓度和血浆时间-浓度曲线下面积在12.5-50.0 mg / kg范围内的剂量不成比例，并且静脉注射后巴豆苷在组织中高度分布。在肝脏中检测到最高的巴豆苷水平（28.79±14.96μg/ g），而在大脑中未检测到巴豆苷。