BACKGROUND The present study investigated the antibacterial activity and underlying mechanisms of ginkgolic acid (GA) C15:1 monomer using green fluorescent protein (GFP)-labeled bacteria strains. RESULTS GA presented significant antibacterial activity against Gram-positive bacteria but generally did not affect the growth of Gram-negative bacteria. The studies of the antibacterial mechanism indicated that large amounts of GA (C15:1) could penetrate GFP-labeled Bacillus amyloliquefaciens in a short period of time, and as a result, led to the quenching of GFP in bacteria. In vitro results demonstrated that GA (C15:1) could inhibit the activity of multiple proteins including DNA polymerase. In vivo results showed that GA (C15:1) could significantly inhibit the biosynthesis of DNA, RNA and B. amyloliquefaciens proteins. CONCLUSION We speculated that GA (C15:1) achieved its antibacterial effect through inhibiting the protein activity of B. amyloliquefaciens. GA (C15:1) could not penetrate Gram-negative bacteria in large amounts, and the lipid soluble components in the bacterial cell wall could intercept GA (C15:1), which was one of the primary reasons that GA (C15:1) did not have a significant antibacterial effect on Gram-negative bacteria.

中文翻译：

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银杏酸C15:1的抗菌活性及机制。


背景本研究使用绿色荧光蛋白（GFP）标记的菌株研究了银杏酸（GA）C15:1单体的抗菌活性和潜在机制。结果 GA对革兰氏阳性菌具有显着的抗菌活性，但一般不影响革兰氏阴性菌的生长。抗菌机制研究表明，大量的GA（C15:1）可以在短时间内穿透GFP标记的解淀粉芽孢杆菌，从而导致细菌内GFP的猝灭。体外结果表明，GA (C15:1) 可以抑制包括 DNA 聚合酶在内的多种蛋白质的活性。体内实验结果表明，GA（C15:1）能够显着抑制DNA、RNA和解淀粉芽孢杆菌蛋白的生物合成。结论我们推测GA(C15:1)通过抑制解淀粉芽孢杆菌的蛋白活性来实现其抗菌作用。 GA(C15:1)不能大量渗透革兰氏阴性菌，细菌细胞壁中的脂溶性成分可以拦截GA(C15:1)，这是GA(C15:1)失效的主要原因之一对革兰氏阴性菌无明显抗菌作用。