This study investigated the influence of nitrogen (N) fertilizer and straw on intact amino acid N uptake by soil microorganisms and the relationship between amino acid turnover and soil properties during the wheat growing season. A wheat pot experiment was carried out with three treatments: control (CK), N fertilizer (NF) and N fertilizer plus rice straw (NS). We used stable isotope compound-specific analysis to determine the uptake of 13C,15N-glycine by soil microorganisms. In the NF treatment, microbial 13C,15N-glycine uptake was lower compared with CK, suggesting that inorganic N was the preferred N source for soil microorganisms. However, The application of straw with N fertilizer (in NS treatment) increased microbial 13C,15N-glycine uptake even with the same amount of N fertilizer application. In this treatment, enzyme activities, soil microbial biomass C and microbial biomass N increased simultaneously because more C was available. Soil mineral N and plant N contents all decreased substantially. The increased uptake of intact 13C,15N-glycine in the NS treatment can be attributed to direct assimilation by soil microorganisms to satisfy the demand for N when inorganic N was consumed.

中文翻译：

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氮肥和秸秆施用会影响小麦生长阶段土壤微生物对13C，15N-甘氨酸的吸收。

本研究调查了氮肥和稻草对小麦生长季土壤微生物对完整氨基酸N吸收的影响以及氨基酸更新与土壤特性之间的关系。小麦盆实验进行了三种处理：对照（​​CK），氮肥（NF）和氮肥加稻草（NS）。我们使用了稳定的同位素化合物特异性分析来确定土壤微生物对13C，15N-甘氨酸的吸收。在NF处理中，微生物13C，15N-甘氨酸的摄取低于CK，这表明无机N是土壤微生物的首选N源。但是，即使在施氮量相同的情况下，施氮肥秸秆（在NS处理中）也会增加微生物对13C，15N-甘氨酸的吸收。在这种治疗中，酶的活性 土壤微生物量碳和微生物量氮同时增加，因为可获得更多的碳。土壤矿质氮和植物氮含量均大幅下降。NS处理中完整的13C，15N-甘氨酸的吸收增加可归因于土壤微生物直接同化，以满足消耗无机N时对N的需求。