9-Oxo-(10E,12E)-octadecadienoic acid (9-EE-KODE), which is isolated from the calyx of eggplants, exhibits cytotoxic activity against human ovarian cancer (HRA) cells. The aim of the present study is to clarify the action mechanism of 9-EE-KODE leading to cell death. After the treatment of 9-EE-KODE in HRA cells, we found intracellular DNA fragmentation, surface-exposure of phosphatidylserine in the outer cell membrane, and increased caspase-3/7 activities in the HRA cells. The dissipation of mitochondrial membrane potential, release of cytochrome c from mitochondria to cytosol, down-regulation of Bcl-2, and up-regulation of Bax levels were also found in 9-EE-KODE-treated cells in a dose-dependent manner. These results demonstrated that 9-EE-KODE induced apoptosis in HRA cells via the mitochondrial regulation pathway.

中文翻译：

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9-Oxo-（10E，12E）-octadecadienoic acid，一种从茄子花萼中分离出的细胞毒性脂肪酸酮二烯，可诱导人卵巢癌（HRA）细胞凋亡。

从茄子的花萼中分离出的9-氧代-（10 E，12 E）-十八碳二烯酸（9- EE -KODE）对人卵巢癌细胞（HRA）表现出细胞毒活性。本研究的目的是阐明9- EE- KODE导致细胞死亡的作用机制。在HRA细胞中处理9- EE -KODE之后，我们发现细胞内DNA片段化，磷脂酰丝氨酸在细胞外膜中的表面暴露以及HRA细胞中caspase-3 / 7活性的增加。在9- EE中还发现线粒体膜电位的耗散，细胞色素c从线粒体释放到细胞质，Bcl-2的下调和Bax的上调-KODE处理的细胞呈剂量依赖性。这些结果证明9- EE- KODE通过线粒体调控途径诱导HRA细胞凋亡。