Isorhamnetin-3-O-neohesperidoside is the major active substance of Puhuang, a traditional herb medicine widely used in clinical practice to tackle many chronic diseases. However, little is known about the interactions between this ingredient and intestinal flora. In this study, ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry together with automated data analysis software (Metabolynx™) was used for analysis of the metabolic profile of isorhamnetin-3-O-neohesperidoside by the isolated human intestinal bacteria. The parent and three metabolites isorhamnetin-3-O-glucoside, isorhamnetin and quercetin were detected and identified based on the characteristics of their deprotonated molecules. These metabolites indicated that isorhamnetin-3-O-neohesperidoside was firstly deglycosylated to isorhamnetin-3-O-glucoside and subsequently to the aglycone isorhamnetin, and the latter was demethylated to quercetin. The majority of bacteria such as Escherichia sp. 23 were capable of converting isorhamnetin-3-O-neohesperidoside to considerable amounts of aglycone isorhamnetin and further to minor amounts of quercetin, while minor amounts of isorhamnetin-3-O-glucoside were detected in minority of bacterial samples such as Enterococcus sp. 30. The metabolic pathway and metabolites of isorhamnetin-3-O-neohesperidoside by the different human intestinal bacteria were firstly investigated. Furthermore, the metabolites of isorhamnetin-3-O-neohesperidoside might influence the effects of traditional herb medicines. Thus, our study is helpful to further unravel how isorhamnetin-3-O-neohesperidoside and Puhuang work in vivo.

中文翻译：

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使用UPLC-Q-TOF / MS的人肠道菌群产生异异鼠李素-3-O-新橙皮苷的代谢谱。

Isorhamnetin-3-O-neohesperidoside是蒲黄的主要活性物质，蒲黄是一种在临床实践中广泛用于应对许多慢性疾病的传统草药。但是，关于这种成分与肠道菌群之间的相互作用了解甚少。在这项研究中，超高效液相色谱/四极杆飞行时间质谱与自动数据分析软件（Metabolynx™）一起用于分析分离的人肠道细菌中异鼠李素-3-O-新橙皮苷的代谢谱。 。根据其去质子化分子的特征，对母体和三种代谢物异鼠李素-3-O-葡萄糖苷，异鼠李素和槲皮素进行了检测和鉴定。这些代谢物表明异鼠李素-3-O-新芥子苷首先被去糖基化为异鼠李素-3-O-葡糖苷，然后被糖苷配基异鼠李素，然后后者被去甲基化为槲皮素。大多数细菌如埃希氏菌属。23个能够将异鼠李素-3-O-新戊二糖苷转化为大量的糖苷异鼠李素，再进一步转化为少量的槲皮素，而在少数细菌样品（如肠球菌sp。）中检测到少量的异鼠李素-3-O-葡糖苷。30.最初研究了异人血红素-3-O-新橙皮苷的不同人肠道细菌的代谢途径和代谢产物。此外，异鼠李素-3-O-新橙皮苷的代谢产物可能会影响传统草药的功效。因此，