基于NaEuF4 / Au @ Ag2S纳米粒子的荧光共振转移DNA传感器，用于超灵敏地检测DNA能量。,Talanta

当前位置： X-MOL 学术 › Talanta › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

基于NaEuF4 / Au @ Ag2S纳米粒子的荧光共振转移DNA传感器，用于超灵敏地检测DNA能量。
Talanta ( IF 5.6 ) Pub Date : 2016-10-23 , DOI: 10.1016/j.talanta.2016.07.051
Yuhong Liu ₁ , Linlin Zhao ₁ , Jin Zhang ₁ , Jinzha Zhang ₁ , Wenbo Zhao ₁ , Chun Mao ₁

Affiliation

这项工作首次研究了使用NaEuF4纳米颗粒（NPs）和Au @ Ag2S NPs作为能量供体-受体对的新型荧光共振能量转移（FRET）系统。基于NaEuF4 / Au @ Ag2S NPs的FRET DNA传感器构建了NaEuF4 NPs作为荧光（FL）供体和Au @ Ag2S核壳NPs作为FL受体。为了找到匹配的能量受体，在合成过程中控制了AgNO3和Na2S的数量，使能量受体的吸收光谱与能量供体的发射光谱重叠。由于Eu3 +由于4f壳的ff电子跃迁而具有较大的斯托克斯位移和窄的发射带，因此可以提高基于FRET的DNA传感器的灵敏度，并降低配体的自吸收以及信号背景。通过研究供体和受体之间的合适距离，我们获得了高效的FRET系统。然后将基于FRET的DNA传感器用于目标DNA的特异性和灵敏检测设计，并且FL的淬灭效率（ΔFL/ F0，ΔFL= F-F0）与目标DNA的浓度呈对数关系，范围从100aM至100pM。我们可以实现目标DNA的超灵敏检测，检测限为32 aM。该方法可用于实际样品中目标DNA的分析，结果令人满意。我们可以实现目标DNA的超灵敏检测，检测限为32 aM。该方法可用于实际样品中目标DNA的分析，结果令人满意。我们可以实现目标DNA的超灵敏检测，检测限为32 aM。该方法可用于实际样品中目标DNA的分析，结果令人满意。

"点击查看英文标题和摘要"

NaEuF4/Au@Ag2S nanoparticles-based fluorescence resonant transfer DNA sensor for ultrasensitive detection of DNA energy.

The work investigates a new fluorescence resonance energy transfer (FRET) system using NaEuF4 nanoparticles (NPs) and Au@Ag2S NPs as the energy donor-acceptor pair for the first time. The NaEuF4/Au@Ag2S NPs-based FRET DNA sensor was constructed with NaEuF4 NPs as the fluorescence (FL) donor and Au@Ag2S core-shell NPs as FL acceptor. In order to find the matching energy acceptor, the amount of AgNO3 and Na2S were controlled in the synthesis process to overlap the absorption spectrum of energy acceptor with the emission spectrum of energy donors. The sensitivity of FRET-based DNA sensor can be enhanced and the self-absorption of ligand as well as the background of signals can be decreased because of Eu3+ which owns large Stokes shifts and narrow emission bands due to f-f electronic transitions of 4f shell. We obtained the efficient FRET system by studying suitable distance between the donor and acceptor. Then the FRET-based DNA sensor was used for the design of specific and sensitive detection of target DNA and the quenching efficiency (ΔFL/F0, ΔFL=F-F0) of FL was logarithmically related to the concentration of the target DNA, ranging from 100aM to 100pM. We can realize an ultrasensitive detection of target DNA with a detection limit of 32 aM. This proposed method was feasible to analyse target DNA in real samples with satisfactory results.

更新日期：2016-08-08

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南