提高西洋参稀有人参皂苷产量的优化微波辅助提取方法。,Journal of Ginseng Research

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提高西洋参稀有人参皂苷产量的优化微波辅助提取方法。
Journal of Ginseng Research ( IF 6.8 ) Pub Date : 2016-10-01 , DOI: 10.1016/j.jgr.2016.06.007
Hua Yao ₁ , Xuwen Li ₂ , Ying Liu ₂ , Qian Wu ₂ , Yongri Jin ₂

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背景西洋参中的稀有人参皂苷具有很强的生物活性。稀有人参皂苷的大量获取十分困难，严重限制了对这些化合物的进一步研究。迫切需要一种简单、快速、有效的方法来同时获取不同种类的稀有人参皂苷并精确定量每种皂苷。方法采用微波辅助提取（MAE）技术从西洋参中提取9种稀有人参皂苷。本文选取稀有人参皂苷[20(S)-Rh1、20(R)-Rh1、Rg6、F4、Rk3、 20(S)-Rg3、20(R)-Rg3、Rk1 和 Rg5] 通过高效液相色谱 (HPLC)-电喷雾电离-质谱法进行鉴定。通过HPLC-UV在203 nm处分析稀有人参皂苷的数量信息。结果 MAE的最佳工艺条件为以水为溶剂，物料比为1:40（w/v），温度145℃，微波功率1,600 W提取15 min。7种稀有人参皂苷[ 20(S)-Rh1、20(R)-Rh1、Rg6、F4、Rk3、Rk1和Rg5]的提取率较高，但20(S)-Rg3和20(R)-Rg3的提取率较低。与常规方法相比，9种稀有人参皂苷的提取率显着提高。结论MAE法可以在短时间内有效地从西洋参中提取稀有人参皂苷。微波辐射在 MAE 中起着重要作用。文章还讨论了稀有人参皂苷的可能生成过程。这对稀有人参皂苷的进一步研究或应用具有重要意义。

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An optimized microwave-assisted extraction method for increasing yields of rare ginsenosides from Panax quinquefolius L.

Background Rare ginsenosides in Panax quinquefolius L. have strong bioactivities. The fact that it is hard to obtain large amounts of rare ginsenosides seriously restricts further research on these compounds. An easy, fast, and efficient method to obtain different kinds of rare ginsenosides simultaneously and to quantify each one precisely is urgently needed. Methods Microwave-assisted extraction (MAE) was used to extract nine kinds of rare ginsenosides from P. quinquefolius L. In this article, rare ginsenosides [20(S)-Rh1, 20(R)-Rh1, Rg6, F4, Rk3, 20(S)-Rg3, 20(R)-Rg3, Rk1, and Rg5] were identified by high performance liquid chromatography (HPLC)–electrospray ionization–mass spectrometry. The quantity information of rare ginsenosides was analyzed by HPLC-UV at 203 nm. Results The optimal conditions for MAE were using water as solvent with the material ratio of 1:40 (w/v) at a temperature of 145°C, and extracting for 15 min under microwave power of 1,600 W. Seven kinds of rare ginsenosides [20(S)-Rh1, 20(R)-Rh1, Rg6, F4, Rk3, Rk1, and Rg5] had high extraction yields, but those of 20(S)-Rg3 and 20(R)-Rg3 were lower. Compared with the conventional method, the extraction yields of the nine rare ginsenosides were significantly increased. Conclusion The results indicate that rare ginsenosides can be extracted effectively by MAE from P. quinquefolius L. in a short time. Microwave radiation plays an important role in MAE. The probable generation process of rare ginsenosides is also discussed in the article. It will be meaningful for further investigation or application of rare ginsenosides.

更新日期：2016-10-01

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