Corydalis yanhusuo, a well-known herbaceous plant, is commonly used in the treatment of inflammation, injury and pain. One natural agent isolated from Corydalis yanhusuo, 13-methyl-palmatrubine, was found to have a cytotoxic effect on cancer cells as reported in published studies. In the present study, we synthesized a potential anti-lung tumor agent, 13-methyl-palmatrubine and analyzed its activity. 13-Methyl-palmatrubine exhibited a cytotoxic effect on a panel of cancer cell lines in a time- and concentration-dependent manner. Among all the tested cancer cell lines, lung cancer A549 cells were most sensitive to 13-methyl-palmatrubine treatment. Meanwhile 13-methyl-palmatrubine showed less cytotoxicity in human normal cells. Our investigation revealed that 13-methyl‑palmatrubine induced apoptosis and cell cycle arrest in A549 cells in a dose-dependent manner. Furthermore, 13-methyl-palmatrubine treatment caused activation of P38 and JNK pathways and blocked the EGFR pathway. In conclusion, our findings demonstrated that 13-methyl-palmatrubine inhibited the growth of A549 cells mediated by blocking of the EGFR signaling pathway and activation of the MAPK signaling pathway and provides a better understanding of the molecular mechanisms of 13-methyl-palmatrubine.

中文翻译：

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13-甲基巴马汀在体外和体内均可诱导A549细胞凋亡和细胞周期停滞。

延胡索（Corydalis yanhusuo）是一种著名的草本植物，通常用于治疗炎症，损伤和疼痛。如已发表的研究报道，发现从延胡索中分离出的一种天然药物13-甲基-palmatrubine对癌细胞具有细胞毒性作用。在本研究中，我们合成了一种潜在的抗肺肿瘤药13-甲基-巴他比林并分析了其活性。13-甲基-palmatrubine以时间和浓度依赖性的方式对一组癌细胞系表现出细胞毒性作用。在所有测试的癌细胞系中，肺癌A549细胞对13-甲基-palmatrubine治疗最为敏感。同时，13-甲基巴马汀在人正常细胞中显示出较小的细胞毒性。我们的研究显示13-甲基-palmatrubine诱导A549细胞凋亡和细胞周期停滞，并呈剂量依赖性。此外，13-甲基-palmatrubine治疗引起P38和JNK通路的激活并阻断EGFR通路。总之，我们的研究结果表明13-甲基-palmatrubine抑制了通过阻断EGFR信号通路和激活MAPK信号通路介导的A549细胞的生长，并提供了对13-甲基-palmatrubine分子机制的更好理解。