Abstract Context 3,4-Oxo-isopropylidene-shikimic acid (ISA) is an analog of shikimic acid (SA). SA is extracted from the dry fruit of Illicium verum Hook. f. (Magnoliaceae), which has been used for treating stomachaches, skin inflammation and rheumatic pain. Objective To investigate the anti-inflammatory, analgesic and antioxidant activities of ISA. Materials and methods Analgesic and anti-inflammatory activities of ISA were evaluated using writhing, hot plate, xylene-induced ear oedema, carrageenan-induced paw oedema and cotton pellets-induced granuloma test, meanwhile the prostaglandin E2 (PGE2) and malondialdehyde (MDA) levels were assessed in the oedema paw tissue. ISA (60, 120 and 240 mg/kg in mice model and 50, 120 and 200 mg/kg in rat model) was administered orally, 30 min before induction of inflammation/pain. Additionally, ISA was administered for 12 d in rats from the day of cotton pellet implantation. The active oxygen species scavenging potencies of ISA (10−3–10−5 M) were evaluated by the electron spin resonance spin-trapping technique. Results ISA caused a reduction of inflammation induced by xylene (18.1–31.4%), carrageenan (7.8–51.0%) and cotton pellets (11.4–24.0%). Furthermore, ISA decreased the production of PGE2 and MDA in the rat paw tissue by 1.0–15.6% and 6.3–27.6%, respectively. ISA also reduced pain induced by acetic acid (15.6–48.9%) and hot plate (10.5–28.5%). Finally, ISA exhibited moderate antioxidant activity by scavenging the superoxide radical and hydroxyl radical with IC50 values of 0.214 and 0.450 μg/mL, respectively. Discussion and conclusion Our findings confirmed the anti-inflammatory, analgesic and antioxidant activities of ISA.

中文翻译：

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3,4-氧代-异亚丙基-莽草酸的抗炎、镇痛和抗氧化活性

摘要上下文3,4-氧代-异亚丙基-莽草酸(ISA)是莽草酸(SA)的类似物。SA 是从 Illicium v​​erum Hook 的干果中提取的。F。（木兰科），用于治疗胃痛、皮肤炎症和风湿痛。目的研究ISA的抗炎、镇痛和抗氧化活性。材料和方法 使用扭体、热板、二甲苯引起的耳部水肿、角叉菜胶引起的爪水肿和棉球引起的肉芽肿试验评估 ISA 的镇痛和抗炎活性，同时使用前列腺素 E2 (PGE2) 和丙二醛 (MDA)在水肿爪组织中评估水平。ISA（小鼠模型中为 60、120 和 240 毫克/千克，大鼠模型中为 50、120 和 200 毫克/千克）在诱导炎症/疼痛前 30 分钟口服给药。此外，从棉球植入之日起，在大鼠中施用 ISA 12 天。通过电子自旋共振自旋捕获技术评估了 ISA (10-3-10-5 M) 的活性氧清除能力。结果 ISA 减少了由二甲苯 (18.1–31.4%)、角叉菜胶 (7.8–51.0%) 和棉球 (11.4–24.0%) 引起的炎症。此外，ISA 使大鼠爪组织中 PGE2 和 MDA 的产生分别降低了 1.0-15.6% 和 6.3-27.6%。ISA 还减轻了由醋酸 (15.6–48.9%) 和热板 (10.5–28.5%) 引起的疼痛。最后，ISA通过清除超氧自由基和羟基自由基表现出中等抗氧化活性，IC50值分别为0.214和0.450 μg/mL。讨论和结论 我们的研究结果证实了 ISA 的抗炎、镇痛和抗氧化活性。