The antioxidant and antidiabetic activity of biomodified alkali lignin extracted from a deciduous plant Acacia nilotica, was evaluated in vitro. The extracted alkali lignin was subjected to microbial biotransformation by ligninolytic fungus Aspergillus flavus and Emericella nidulans. These modifications were done under varying concentration of carbon to nitrogen sources. The structural feature of the lignin samples were compared by FTIR, functional group analysis and (13)C solid state NMR. All lignin samples were tested for antioxidant efficiency, reducing power and H2O2 scavenging power. Modifications in all lignin samples showed correlation with their antioxidant scavenging activity and reducing power. Antidiabetic properties were evaluated in terms of in vitro glucose movement inhibition and α-amylase inhibition assay. Modified samples exhibited increased glucose binding efficiency as demonstrated by the decreased glucose diffusion (55.5-76.3%) and 1.16-1.18-fold enhanced α-amylase inhibition in comparison to their control samples. The results obtained demonstrate that the structure and functional modifications in lignin significantly affects its bioefficacy in term of antioxidant and antidiabetic activities.

中文翻译：

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相思木生物改性木质素的体外抗氧化和抗糖尿病活性。

在体外评估了从落叶植物金合欢中提取的生物改性碱木质素的抗氧化和抗糖尿病活性。提取的碱木质素通过木质素分解真菌黄曲霉和构巢杜鹃（Emericella nidulans）进行微生物生物转化。这些修饰是在碳与氮源浓度变化的情况下完成的。通过FTIR，官能团分析和（13）C固态NMR比较了木质素样品的结构特征。测试了所有木质素样品的抗氧化剂效率，还原能力和H2O2清除能力。所有木质素样品中的修饰物均与其抗氧化剂清除活性和还原能力相关。通过体外葡萄糖运动抑制和α-淀粉酶抑制试验评估抗糖尿病特性。与对照样品相比，修饰的样品表现出增加的葡萄糖结合效率，这通过减少的葡萄糖扩散（55.5-76.3％）和1.16-1.18倍增强的α-淀粉酶抑制来证明。获得的结果表明，木质素的结构和功能修饰在抗氧化剂和抗糖尿病活性方面显着影响其生物功效。