Facile and simple processes to get Zn-Fe layered double hydroxide (LDH) with nitrate as the interlayer anion are reported. The method of co-precipitation produced high crystallinity LDH that is marked by XRD, SEM, TEM and FT-IR. Results showed that 99.8% of Cd^+ 2 removals were at pH 11 and 4 h. To get the adsorption isotherms, the concentration of metal ions extending from 6 to 18 mg/L was utilized. Results supported the Langmuir adsorption model. In contrary, the adsorption process followed the pseudo-second-order reaction kinetics. Interestingly, the prepared LDH shows durable antimicrobial activities against Gram-negative (Proteous vulgaris, Klebsiella pneumoniae, Escherichia coli and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus epidermidis, Staphylococcus aureus, Streptococcus pyogenes and MRSA) and fungi (Candida albicans, Aspergillus fumigatus, Geotricumcandidum, and Trichophyton mentagrophytes). The minimum inhibitory concentration (MIC) of Zn-Fe LDH varied from 0.49 to 15.60 μg/mL according to the types of microorganisms. The prepared LDH achieved 90% at pH 8.50 which is the pH of wastewater and at the same time exhibited durable antimicrobial activities against MRSA, Gram-negative, Gram-positive and fungi. Results have significant implications in the field of bioremediation of water with little cost, simple operation, high productivity and easiness of the equipment.