Parishin is a phenolic glucoside isolated from Gastrodia elata, which is an important traditional Chinese medicine; this glucoside significantly extended the replicative lifespan of K6001 yeast at 3, 10, and 30 μM. To clarify its mechanism of action, assessment of oxidative stress resistance, superoxide dismutase (SOD) activity, malondialdehyde (MDA), and reactive oxygen species (ROS) assays, replicative lifespans of sod1, sod2, uth1, and skn7 yeast mutants, and real-time quantitative PCR (RT-PCR) analysis were conducted. The significant increase of cell survival rate in oxidative stress condition was observed in parishin-treated groups. Silent information regulator 2 (Sir2) gene expression and SOD activity were significantly increased after treating parishin in normal condition. Meanwhile, the levels of ROS and MDA in yeast were significantly decreased. The replicative lifespans of sod1, sod2, uth1, and skn7 mutants of K6001 yeast were not affected by parishin. We also found that parishin could decrease the gene expression of TORC1, ribosomal protein S26A (RPS26A), and ribosomal protein L9A (RPL9A) in the target of rapamycin (TOR) signaling pathway. Gene expression levels of RPS26A and RPL9A in uth1, as well as in uth1, sir2 double mutants, were significantly lower than those of the control group. Besides, TORC1 gene expression in uth1 mutant of K6001 yeast was inhibited significantly. These results suggested that parishin exhibited antiaging effects via regulation of Sir2/Uth1/TOR signaling pathway.

中文翻译：

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天麻的Parishin通过调节Sir2 / Uth1 / TOR信号通路来延长酵母的寿命。

香蒲素是从天麻中分离出来的一种酚类葡萄糖苷，天麻是一种重要的中药。在这3糖苷显著扩展K6001酵母的复制寿命，10，和30  μ M.为了阐明其作用机理，氧化胁迫抗性的评价，超氧化物歧化酶（SOD）活性，丙二醛（MDA），以及活性氧簇（进行了ROS）测定，sod1，sod2，uth1和skn7酵母突变体的复制寿命，以及实时定量PCR（RT-PCR）分析。在帕里申处理的组中观察到在氧化应激条件下细胞存活率的显着增加。静默信息调节器2（在正常条件下处理parishin后，Sir2）基因表达和SOD活性显着增加。同时，酵母中的ROS和MDA水平显着降低。K6001酵母的sod1，sod2，uth1和skn7突变体的复制寿命不受parishin影响。我们还发现，parishin可以降低雷帕霉素（TOR）信号通路靶标中的TORC1，核糖体蛋白S26A（RPS26A）和核糖体蛋白L9A（RPL9A）的基因表达。基因表达水平RPS26A和RPL9A中值U TH1，以及在值U TH1，SIR2双突变体，均显著低于对照组的降低。此外，TORC1基因在K6001酵母uth1突变体中的表达也被显着抑制。这些结果表明，parishin通过调节Sir2 / Uth1 / TOR信号通路表现出抗衰老作用。