亚硝酸盐/硝酸盐培养的反硝化除磷污泥长期厌氧饥饿过程中内源性代谢的比较。,Water Research

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亚硝酸盐/硝酸盐培养的反硝化除磷污泥长期厌氧饥饿过程中内源性代谢的比较。
Water Research ( IF 11.4 ) Pub Date : 2014-12-03 , DOI: 10.1016/j.watres.2014.09.044
Yayi Wang ₁ , Shuai Zhou , Hong Wang , Liu Ye , Jian Qin , Ximao Lin

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通过使富磷生物（DPAO）反硝化来去除磷（DPR）是减少能源和碳消耗的一种有前途的方法。但是，进水的波动或干扰经常使DPAO的生物量处于饥饿状态，降低了生物量的活性和数量，并最终降低了DPR的性能。因此，迫切需要对DPAOs的内源性代谢和恢复能力有更好的了解。在本研究中，在亚硝酸盐和硝酸盐培养的DPAO中，在20±1°C下研究了厌氧饥饿（12天）和恢复情况。从厌氧和好氧阶段结束开始，亚硝酸盐-DPAOs污泥的细胞衰变率分别为0.008天-1和0.007天-1，比硝酸盐-DPAOs污泥的细胞衰减率分别低64％和68％。饥饿12天后，亚硝酸盐-DPAOs污泥的回收率也比硝酸盐-DPAOs污泥恢复得更快。从厌氧和好氧阶段开始，亚硝酸盐-DPAOs污泥的维持能量分别比硝酸盐-DPAOs污泥的维持能量低约31％和34％。在亚硝酸盐和硝酸盐-DPAOs污泥中，糖原和聚磷酸盐（poly-P）依次成为主要的维持能源。然而，饥饿期间细胞内聚合物的转化途径在它们之间是不同的。在饥饿的前三天内，硝酸盐-DPAOs污泥使用细胞外聚合物（EPS）（主要是多糖）作为额外的维持能源。在此阶段，EPS似乎占硝酸盐-DPAOs中ATP产量的19-27％，但对亚硝酸盐-DPAOs的细胞维持作用要小得多。亚硝酸盐-DPAO对饥饿的高抵抗力可能归因于频繁的短期饥饿和在母体亚硝酸盐喂养的反应堆中暴露于有毒物质，例如亚硝酸盐/游离亚硝酸。亚硝酸盐-DPAOs污泥对厌氧饥饿的强大抵抗力可通过短程反硝化过程去除磷。

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Comparison of endogenous metabolism during long-term anaerobic starvation of nitrite/nitrate cultivated denitrifying phosphorus removal sludges.

Denitrifying phosphorus removal (DPR) by denitrifying phosphorus-accumulating organisms (DPAOs) is a promising approach for reducing energy and carbon usage. However, influent fluctuations or interruptions frequently expose the DPAOs biomass to starvation conditions, reducing biomass activity and amount, and ultimately degrading the performance of DPR. Therefore, a better understanding of the endogenous metabolism and recovery ability of DPAOs is urgently required. In the present study, anaerobic starvation (12 days) and recovery were investigated in nitrite- and nitrate-cultivated DPAOs at 20 ± 1 °C. The cell decay rates in nitrite-DPAOs sludges from the end of the anaerobic and aerobic phase were 0.008 day⁻¹ and 0.007 day⁻¹, respectively, being 64% and 68% lower than those of nitrate-DPAOs sludges. Nitrite-DPAOs sludges also recovered more rapidly than nitrate-DPAOs sludge after 12 days of starvation. The maintenance energy of nitrite-DPAOs sludges from the end of the anaerobic and aerobic phase were approximately 31% and 34% lower, respectively, than those of nitrate-DPAOs sludges. Glycogen and polyphosphate (poly-P) sequentially served as the main maintenance energy sources in both nitrite-and nitrate-DPAOs sludges. However, the transformation pathway of the intracellular polymers during starvation differed between them. Nitrate-DPAOs sludge used extracellular polymeric substances (EPS) (mainly polysaccharides) as an additional maintenance energy source during the first 3 days of starvation. During this phase, EPS appeared to contribute to 19-27% of the ATP production in nitrate-DPAOs, but considerably less to the cell maintenance of nitrite-DPAOs. The high resistance of nitrite-DPAOs to starvation might be attributable to frequent short-term starvation and exposure to toxic substances such as nitrite/free nitrous acids in the parent nitrite-fed reactor. The strong resistance of nitrite-DPAOs sludge to anaerobic starvation may be exploited in P removal by shortcut denitrification processes.

更新日期：2019-11-01

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