人胎儿胸腺中细胞角蛋白（CK5，CK8，CK14）的表达和祖干细胞的存在。,Clinical Anatomy

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人胎儿胸腺中细胞角蛋白（CK5，CK8，CK14）的表达和祖干细胞的存在。
Clinical Anatomy ( IF 2.3 ) Pub Date : 2016-05-24 , DOI: 10.1002/ca.22736
Richa Gupta ₁ , Tulika Gupta ₁ , Harjeet Kaur ₁ , Shobha Sehgal ₂ , Anjali Aggarwal ₁ , Kanchan Kapoor ₃ , Anshu Sharma ₃ , Daisy Sahni ₁ , Suhalika Singla ₄

Affiliation

本研究的目的是观察人胎儿胸腺中细胞角蛋白的表达。在成年人类和小鼠中，特定的细胞角蛋白标记已被很好地描述，但对人类胎儿的研究却很少。我们还旨在观察胎儿胸腺组织中是否存在可以收获来治疗各种免疫缺陷疾病的祖细胞。从30名流产的人类胎儿（12至31周）获得的胸腺经过免疫组织化学检查，以研究不同胎龄上皮细胞（TEC）中细胞角蛋白表达的变化。在妊娠16周之前，皮质（cTEC）和髓质（mTEC）TEC表现出细胞角蛋白CK8和CK5的均质染色。16周后，出现差异染色，其中cTEC对CK8呈阳性，而mTEC对CK5和CK14呈阳性。有趣的是，CK5 + CK8 +祖干细胞在所有胎龄均存在于胎儿胸腺皮质中，在12至16周内相对较高。胎儿胸腺中细胞角蛋白的表达与成年胸腺中的差异很大，这是因为胎儿胸腺基质中存在未分化的祖干细胞以及分化的TEC。从胎儿胸腺基质中收集这些祖干细胞以治疗各种免疫缺陷疾病的最佳时间似乎是12-16周。临床阿纳特 29：711-717，2016.©2016 Wiley Periodicals，Inc. 胎儿胸腺中细胞角蛋白的表达与成年胸腺中的差异很大，这是由于胎儿胸腺基质中存在未分化的祖干细胞以及分化的TEC。从胎儿胸腺基质中收集这些祖干细胞以治疗各种免疫缺陷疾病的最佳时间似乎是12-16周。临床阿纳特 29：711-717，2016.©2016 Wiley Periodicals，Inc. 胎儿胸腺中细胞角蛋白的表达与成年胸腺中的差异很大，这是因为胎儿胸腺基质中存在未分化的祖干细胞以及分化的TEC。从胎儿胸腺基质中收集这些祖干细胞以治疗各种免疫缺陷疾病的最佳时间似乎是12-16周。临床阿纳特 29：711-717，2016.©2016 Wiley Periodicals，Inc.

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Cytokeratin (CK5, CK8, CK14) expression and presence of progenitor stem cells in human fetal thymuses.

The aim of the current study was to observe the expression of cytokeratins in human fetal thymuses. Specific cytokeratin markers in adult humans and mice have been well described but there has been little similar work on human fetuses. We also aimed to see whether progenitor stem cells that could be harvested to treat various immunodeficiency disorders are present in fetal thymic tissue. Thymuses obtained from 30 aborted human fetuses (12 to 31 weeks) were examined immunohistochemically to investigate changes in cytokeratin expression in the epithelial cells (TEC) at various gestational ages. Before 16 weeks of gestation, cortical (cTEC) and medullary (mTEC) TEC exhibited homogenous staining for cytokeratins CK8 and CK5. After 16 weeks there was differential staining, with cTEC positive for CK8 and mTEC for CK5 and CK14. Interestingly, both CK5 + CK8+ progenitor stem cells were present in the fetal thymic cortex at all gestational ages, with a relatively high number from 12 to 16 weeks. Cytokeratin expression in fetal thymuses was quite different from that in the adult thymus owing to the presence of undifferentiated progenitor stem cells in fetal thymic stroma along with differentiated TEC. The best time to harvest these progenitor stem cells from fetal thymic stroma in order to treat various immune deficiency disorders appears to be 12-16 weeks. Clin. Anat. 29:711-717, 2016. © 2016 Wiley Periodicals, Inc.

更新日期：2019-11-01

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