装有QK肽的电纺PELCL膜可增强血管内皮细胞的生长。,Journal of Materials Science: Materials in Medicine

当前位置： X-MOL 学术 › J. Mater. Sci. Mater. Med. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

装有QK肽的电纺PELCL膜可增强血管内皮细胞的生长。
Journal of Materials Science: Materials in Medicine ( IF 4.2 ) Pub Date : 2016-04-25 , DOI: 10.1007/s10856-016-5705-6
Yang Yang ₁ , Qingmao Yang ₂ , Fang Zhou ₁ , Yunhui Zhao ₁ , Xiaoling Jia ₂ , Xiaoyan Yuan ₁ , Yubo Fan _{2,

3}

Affiliation

小直径血管移植物组织工程的主要挑战之一是抑制内膜增生并在植入后保持长期通畅。移植物的快速内皮化可能是一种有效的方法。在这项研究中，选择QK（一种模拟血管内皮生长因子的肽）作为生物活性底物，并装入电纺膜中以增强血管内皮细胞的生长。详细地说，首先将QK肽与聚乙二醇二丙烯酸酯一起引入可以转移到水凝胶中的硫醇化壳聚糖溶液中。然后，将含有QK肽的聚（乙二醇）-b-聚（L-丙交酯-ε-己内酯）（PELCL）（有或没有壳聚糖水凝胶）的悬浮液或乳液电纺成纤维膜。为了比较，还制造了没有QK的静电纺PELCL膜。释放行为的结果表明，静电纺丝膜，尤其是含有通过悬浮静电纺丝制备的壳聚糖水凝胶的膜，可以成功地封装QK肽并在释放后保持其二级结构。体外细胞培养研究表明，QK肽的释放可在9天之内加速血管内皮细胞的增殖。提示载有QK肽的电纺PELCL膜可能在血管组织工程中具有潜在的应用。可以成功地封装QK肽并在释放后保持其二级结构。体外细胞培养研究表明，QK肽的释放可在9天之内加速血管内皮细胞的增殖。提示载有QK肽的电纺PELCL膜可能在血管组织工程中具有潜在的应用。可以成功地封装QK肽并在释放后保持其二级结构。体外细胞培养研究表明，QK肽的释放可在9天之内加速血管内皮细胞的增殖。提示载有QK肽的电纺PELCL膜可能在血管组织工程中具有潜在的应用。

"点击查看英文标题和摘要"

Electrospun PELCL membranes loaded with QK peptide for enhancement of vascular endothelial cell growth.

One of the major challenges in tissue engineering of small-diameter vascular grafts is to inhibit intimal hyperplasia and keep long-term patency after implantation. Rapid endothelialization of the grafts could be an effective approach. In this study, QK, a peptide mimicking vascular endothelial growth factor, was selected as the bioactive substrate and loaded in electrospun membranes for enhancement of vascular endothelial cell growth. In detail, QK peptide was firstly introduced with poly(ethylene glycol) diacrylate into a thiolated chitosan solution that could transfer into hydrogel. Then, suspensions or emulsions of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) (PELCL) containing QK peptide (with or without chitosan hydrogel) were electrospun into fibrous membranes. For comparison, the electrospun PELCL membrane without QK was also fabricated. Results of release behaviors showed that the electrospun membranes, especially that contained chitosan hydrogel prepared by suspension electrospinning, could successfully encapsulate QK peptide and maintain its secondary structure after released. In vitro cell culture studies exhibited that the release of QK peptide could accelerate the proliferation of vascular endothelial cells in the 9 days. It was suggested that the electrospun PELCL membranes loaded with QK peptide might have potential applications in vascular tissue engineering.

更新日期：2019-11-01

点击分享查看原文

点击收藏

阅读更多本刊新发论文