Confocal live imaging of the amphiphilic styryl dye FM4-64 is a valuable technique to monitor organelle dynamics and in particular endocytic pathways. After application in plants, FM4-64 immediately stains the plasma membrane and is then integrated on vesicles following endomembrane system-dependent internalization processes. Over time, FM4-64 becomes distributed throughout the full vesicular network from the plasma membrane to the vacuole, including the components of the secretory pathways. Here we provide succinct examples of the many important developmental processes in plants that rely on endocytosis and describe two suitable methods to trace the endocytic pathways in Arabidopsis thaliana root cells based on the uptake of FM4-64.

中文翻译：

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FM4-64的活细胞成像，一种追踪拟南芥根细胞内吞途径的工具。

两亲性苯乙烯基染料FM4-64的共聚焦实时成像是一种有价值的技术，可监测细胞器动力学，尤其是内吞途径。在植物中施用后，FM4-64立即染色质膜，然后在依赖于内膜系统的内化过程中整合到囊泡上。随着时间的流逝，FM4-64从质膜到液泡遍布整个囊泡网络，包括分泌途径的成分。在这里，我们提供了依赖内吞作用的植物中许多重要发育过程的简要示例，并描述了两种基于FM4-64吸收来追踪拟南芥根细胞内吞途径的合适方法。