Trh（tdh- / trh +）对副溶血性弧菌的临床，环境和食品分离株的基因分析，作为调查致病性的工具。,International Journal of Food Microbiology

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Trh（tdh- / trh +）对副溶血性弧菌的临床，环境和食品分离株的基因分析，作为调查致病性的工具。
International Journal of Food Microbiology ( IF 5.0 ) Pub Date : 2016-03-19 , DOI: 10.1016/j.ijfoodmicro.2016.02.016
Francesca Leoni ₁ , Giulia Talevi ₁ , Laura Masini ₁ , Donatella Ottaviani ₁ , Elena Rocchegiani ₁

Affiliation

从2002年至2011年在意大利从临床，环境和食品样本中分离的tdh- / trh +副溶血性弧菌的TDH相关溶血素的trh基因的测序分析揭示了所有分离物中都存在trh2变体。临床分离株的trh2与来自欧洲的其他临床tdh- / trh2副溶血性弧菌有100％相同。来自意大利和其他国家的临床分离株的trh2序列中核苷酸和氨基酸的差异，使临床菌株与意大利分离出的大多数环境或食品菌株有所区别。来自欧洲（意大利，挪威和德国）的临床菌株中存在113和115位的天冬氨酸和异亮氨酸，由完整的trh基因序列的337-339位和343-345位核苷酸三联体GAT和ATT编码，亚洲和美国。来自意大利的环境或食物来源的tdh- / trh2副溶血性弧菌有35.5％共享临床分离株中检测到的三胞胎/氨基酸，而来自海洋环境的分离株中有64.5％与临床来源分离，这表明在来自环境的菌株的trh2序列之间会出现差异，这些多态性可能会将潜在的致病菌与环境和海鲜中较少或无病原性的培养物区分开。另外，在这组来自不同来源的tdh- / trh +副溶血性弧菌和三种临床tdh + / trh-副溶血性弧菌分离株中研究了T3SS2基因的分布。所有tdh- / trh + V. parahaemolyticus的环境或食物来源，与孤立年份或地理来源无关，扩增了所有筛选的T3SS2β基因，并对所有5个T3SS2α基因的PCR检测均呈阴性，这是tdh- / trh +临床副溶血性弧菌的分离物。对编码T3SS2效应蛋白之一的vopC基因进行了部分测序，并与来自其他国家的临床tdh- / trh +和tdh + / trh +副溶血性弧菌分离株进行了比较。对T3SS2βvopC序列的分析显示，来自意大利的tdh- / trh2分离株具有变异性，这些分离株与源自美国trh2副溶血性弧菌的一组vopC序列分离。副溶血性疟原虫来自其他国家。对T3SS2βvopC序列的分析显示，来自意大利的tdh- / trh2分离株存在变异，该分离株与源自美国trh2副溶血性弧菌的一组vopC序列分离。副溶血性疟原虫来自其他国家。对T3SS2βvopC序列的分析显示，来自意大利的tdh- / trh2分离株存在变异，该分离株与源自美国trh2副溶血性弧菌的一组vopC序列分离。

"点击查看英文标题和摘要"

Trh (tdh-/trh+) gene analysis of clinical, environmental and food isolates of Vibrio parahaemolyticus as a tool for investigating pathogenicity.

Sequencing analysis of the trh gene encoding the TDH-related haemolysin of tdh-/trh+ Vibrio parahaemolyticus isolated in Italy between 2002 and 2011 from clinical, environmental, and food samples revealed the presence of the trh2 variant in all isolates. The trh2 of the clinical isolate was 100% identical to other clinical tdh-/trh2 V. parahaemolyticus from Europe. Nucleotide and amino acid differences in the trh2 sequences of clinical isolates from Italy and other countries allowed a differentiation of the clinical strains from the majority of environmental or food strains isolated in Italy. Aspartic acid and isoleucine at positions 113 and 115, encoded by nucleotide triplets GAT and ATT at positions 337-339 and 343-345 of the complete trh gene sequence, were present in clinical strains from Europe (Italy, Norway and Germany), Asia and the United States. Only 35.5% of the tdh-/trh2 V. parahaemolyticus of environmental or food origin from Italy shared the same triplets/amino acid detected in clinical isolates, while 64.5% of isolates from the marine environment were different from those of clinical origins, demonstrating that differences occur amongst the trh2 sequences of strains from the environment and these polymorphisms may differentiate potentially pathogenic from less or non-pathogenic cultures found in the environment and seafood. In addition the distribution of T3SS2 genes was investigated in this group of tdh-/trh+ V. parahaemolyticus from different sources and in three clinical tdh+/trh- V. parahaemolyticus isolates. All tdh-/trh+ V. parahaemolyticus of environmental or food source, independent of year of isolation or geographical origin, amplified all the screened T3SS2β genes and tested negative to PCR assays for all five T3SS2α genes, as the tdh-/trh+ clinical V. parahaemolyticus isolate. The vopC genes, encoding for one of the effector proteins of T3SS2, were partially sequenced and compared to clinical tdh-/trh+ and tdh+/trh+ V. parahaemolyticus isolates from other countries. Analysis of T3SS2β vopC sequences revealed variation in tdh-/trh2 isolates from Italy, which were separated from a group of vopC sequences derived from trh2 V. parahaemolyticus from the USA.

更新日期：2016-03-02

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