重组成纤维细胞生长因子（Q40P / S47I / H93G rFGF-1）在抗衰老治疗中的活性和有效性的研究。,Advances in Dermatology and Allergology

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重组成纤维细胞生长因子（Q40P / S47I / H93G rFGF-1）在抗衰老治疗中的活性和有效性的研究。
Advances in Dermatology and Allergology Pub Date : 2016-03-18 , DOI: 10.5114/pdia.2014.44024
Justyna Żerańska ₁ , Monika Pasikowska ₂ , Barbara Szczepanik ₂ , Krzysztof Mlosek ₃ , Sylwia Malinowska ₃ , Renata M Dębowska ₄ , Irena Eris ₄

Affiliation

简介成纤维细胞生长因子1（FGF-1）是一种强大的促分裂原，参与刺激DNA合成和多种细胞类型的增殖。对成纤维细胞生长因子1进行了基因修饰，以提高其热稳定性和对蛋白酶降解的抵抗力，而不会失去其生物活性。目的研究Q40P / S47I / H93G rFGF-1对皮肤细胞的影响，其对皮肤的渗透作用以及对rFGF-1-化妆品产品特性的评估。材料与方法体外研究包括与rFGF-1孵育后检查原代成纤维细胞和角质形成细胞的活力。通过拉曼光谱离体检查了rFGF-1在各种制剂和载体系统中的渗透能力。体内研究-HF超声和3D成像系统-用于评估含有rFGF-1的面霜的抗衰老特性。结果体外研究表明，rFGF-1可以显着增强被处理细胞的生存能力。拉曼光谱分析表明，包裹在脂质球体中的rFGF-1穿透角质层的深度为60 µm，并添加到o / w配方中-可以穿透的深度为90 µm。从Primos获得的结果表明皱纹的体积和深度减少了。通过HF超声检查分析区域中皮肤结构的变化。结论重组FGF-1强烈刺激成纤维细胞和角质形成细胞增殖。但是，这种蛋白质通过SC的过渡需要适当的载体系统-脂质球。

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A study of the activity and effectiveness of recombinant fibroblast growth factor (Q40P/S47I/H93G rFGF-1) in anti-aging treatment.

INTRODUCTION Fibroblast growth factor 1 (FGF-1) is a powerful mitogen involved in the stimulation of DNA synthesis and the proliferation of a wide variety of cell types. Fibroblast growth factor 1 was genetically modified to improve its thermal stability and resistance to protease degradation without losing its biological activity. AIM To study the impact of Q40P/S47I/H93G rFGF-1 on skin cells, its penetration through the skin and the evaluation of the rFGF-1-cosmetic product properties. MATERIAL AND METHODS In vitro studies included the examination of primary fibroblast and keratinocyte viability after the incubation with rFGF-1. The penetration abilities of rFGF-1 in various formulations and carrier systems were examined ex vivo by the Raman spectroscopy. In vivo studies - HF Ultrasound and 3D Imaging System - were used to evaluate the anti-aging properties of creams containing rFGF-1. RESULTS In vitro studies demonstrated that rFGF-1 strongly enhanced the viability of the treated cells. The Raman Spectroscopy analysis indicated that rFGF-1 encapsulated in lipid spheres penetrate through the stratum corneum to the depth of 60 µm, and added to the o/w formulation - could penetrate to a depth of 90 µm. The results obtained from Primos revealed the reduction of the volume and the depth of the wrinkles. Changes in the skin structure in the analyzed areas were evaluated by HF Ultrasonography. CONCLUSIONS Recombinant FGF-1 strongly stimulated fibroblast and keratinocyte proliferation. However, the transition of this protein through the SC required an appropriate carrier system - lipid spheres. All tests - in vitro, ex vivo and in vivo - have proved that rFGF-1 is a substance with a potentially wide spectrum of use.

更新日期：2019-11-01

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