Background Ginsenosides, the major ingredients of Panax ginseng, have been studied for many decades in Asian countries as a result of their wide range of pharmacological properties. The less polar ginsenosides, with one or two sugar residues, are not present in nature and are produced during manufacturing processes by methods such as heating, steaming, acid hydrolysis, and enzyme reactions. 1H-NMR and 13C-NMR spectroscopic data for the identification of the less polar ginsenosides are often unavailable or incomplete. Methods We isolated 21 compounds, including 10 pairs of 20(S) and 20(R) less polar ginsenosides (1–20), and an oleanane-type triterpene (21) from a processed ginseng preparation and obtained complete 1H-NMR and 13C-NMR spectroscopic data for the following compounds, referred to as compounds 1–21 for rapid identification: 20(S)-ginsenosides Rh2 (1), 20(R)-Rh2 (2), 20(S)-Rg3 (3), 20(R)-Rg3 (4), 6′-O-acetyl-20(S)-Rh2 [20(S)-AcetylRh2] (5), 20(R)-AcetylRh2 (6), 25-hydroxy-20(S)-Rh2 (7), 25-hydroxy-20(S)-Rh2 (8), 20(S)-Rh1 (9), 20(R)-Rh1 (10), 20(S)-Rg2 (11), 20(R)-Rg2 (12), 25-hydroxy-20(S)-Rh1 (13), 25-hydroxy-20(R)-Rh1 (14), 20(S)-AcetylRg2 (15), 20(R)-AcetylRg2 (16), Rh4 (17), Rg5 (18), Rk1 (19), 25-hydroxy-Rh4 (20), and oleanolic acid 28-O-β-D-glucopyranoside (21).

中文翻译：

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20(S) 和 20(R) 人参皂苷对的完整 1H-NMR 和 13C-NMR 光谱分析

背景人参皂苷是人参的主要成分，由于其广泛的药理特性，亚洲国家已对其进行了数十年的研究。极性较小的人参皂苷，带有一两个糖残基，在自然界中并不存在，而是在制造过程中通过加热、蒸煮、酸水解和酶反应等方法产生的。用于鉴定极性较小的人参皂苷的 1H-NMR 和 13C-NMR 光谱数据通常不可用或不完整。方法 我们从加工过的人参制剂中分离出 21 种化合物，包括 10 对 20(S) 和 20(R) 极性较小的人参皂苷 (1–20) 和齐墩果型三萜 (21)，并获得完整的 1H-NMR 和 13C - 以下化合物的 NMR 光谱数据，称为化合物 1-21，用于快速鉴定：