We synthesized 2,3,6,7,10,11-hexahydroxytriphenylene (HHTP), characterized it by electrochemistry, spectroelectrochemistry, and electron paramagnetic resonance techniques, and evaluated its cytotoxicity to human cancer cell lines. The results revealed that HHTP has accessible higher-oxidation states, especially the tris-semiquinone monoradical. This species is stable and is formed after being stored for months. HHTP exhibited cytotoxic effects on 5 human cancer cell lines, including glioma and lung cancer cells. The cytotoxic effect was evaluated based on the decrease in cell viability, increases in the percentage of cells with fragmented DNA, and elevated numbers of annexin V-PI-positive cells after HHTP treatment.

中文翻译：

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2,3,6,7,10,11-六羟基联苯的表征及其对人癌细胞系中细胞活力的影响。

我们合成了2,3,6,7,10,11-六羟基三亚苯基（HHTP），通过电化学，光谱电化学和电子顺磁共振技术对其进行了表征，并评估了其对人类癌细胞系的细胞毒性。结果表明，HHTP具有较高的氧化态，尤其是三-半醌单自由基。该物种是稳定的，并且在储存数月后形成。HHTP对5种人类癌细胞系（包括神经胶质瘤和肺癌细胞）表现出细胞毒性作用。根据HHTP处理后细胞活力的降低，DNA片段化细胞百分比的增加以及膜联蛋白V-PI阳性细胞数量的增加来评估细胞毒性作用。