Two photo‐crosslinking biarsenical (CrAsH‐EDT2)‐modified probes were synthesized that are expected to be useful tools for tetracysteine‐labeled proteins to facilitate the co‐affinity purification of their DNA binding sequences and interacting proteins. In addition, improvements for the synthesis of CrAsH‐EDT2 and N1‐(4‐azido‐2‐nitrophenyl)hexane‐1,6‐diamine are reported. Both photoprobes effectively entered HeLa cells (and the nucleus) and were dependent on the tetracysteine motif in recombinant DMRT1 (doublesex and Mab3‐related transcription factor) to induce fluorescence, suggesting that their crosslinking abilities can be exploited for the identification of nucleic acids and proteins associated with a protein of interest.

中文翻译：

---

合成含有芳基叠氮和二氮丙啶的 CrAsH-EDT2 光亲和探针

合成了两种光交联双砷（CrAsH-EDT2）修饰的探针，有望成为四半胱氨酸标记蛋白质的有用工具，以促进其 DNA 结合序列和相互作用蛋白质的共亲和纯化。此外，还报道了对 CrAsH-EDT2 和 N1-(4-叠氮基-2-硝基苯基)己烷-1,6-二胺合成的改进。两种光探针都有效地进入 HeLa 细胞（和细胞核），并依赖于重组 DMRT1（doublesex 和 Mab3 相关转录因子）中的四半胱氨酸基序来诱导荧光，表明它们的交联能力可用于鉴定核酸和蛋白质与感兴趣的蛋白质相关。