Chronic obstructive pulmonary disease (COPD) is mainly caused by cigarette smoking and is characterized by the destruction of lung parenchyma, structural alterations of the small airways, and systemic inflammation. Tuberostemonine (TS) is an alkaloid-type phytochemical from Stemona tuberosa. In the present study, we evaluated the anti-inflammatory effect of TS in a cigarette smoke (CS)-induced mouse model of acute lung inflammation. The mice were whole-body exposed to CS or fresh air for 7 days. TS was administered by an intraperitoneal (i.p.) injection 1h before exposure to CS. To test the effects of TS, the numbers of total cells, neutrophils, macrophages and lymphocytes in the bronchoalveolar lavage (BAL) fluid were counted. Furthermore, we measured the levels of several chemokines, such as GCP-2, MIP-3α, MCP-1 and KC, in the lung tissue. The cellular profiles and histopathological analysis demonstrated that the infiltration of peribronchial and perivascular inflammatory cells significantly decreased in the TS-treated groups compared with the CS-exposure group. The TS treatment significantly ameliorated the airway epithelial thickness induced by CS exposure and caused a significant decrement in the production of chemokines in the lung. These results suggest that TS has anti-inflammatory effects against CS-induced acute lung inflammation.

中文翻译：

---

结核菌丁宁对香烟烟雾引起的小鼠急性肺部炎症的治疗作用。

慢性阻塞性肺疾病（COPD）主要由吸烟引起，其特征是肺实质破坏，小气道结构改变和全身性炎症。Tuberostemonine（TS）是一种来自Stemona tuberosa的生物碱型植物化学物质。在本研究中，我们评估了TS在香烟烟雾（CS）诱导的急性肺部炎症小鼠模型中的抗炎作用。使小鼠全身暴露于CS或新鲜空气中7天。暴露于CS前1h通过腹膜内（ip）注射给予TS。为了测试TS的作用，计算了支气管肺泡灌洗液（BAL）中总细胞，嗜中性粒细胞，巨噬细胞和淋巴细胞的数量。此外，我们测量了肺组织中几种趋化因子的水平，例如GCP-2，MIP-3α，MCP-1和KC。细胞概况和组织病理学分析表明，与CS暴露组相比，TS治疗组的支气管周和血管周炎性细胞浸润明显减少。TS治疗显着改善了CS暴露引起的气道上皮厚度，并导致肺趋化因子的产生显着减少。这些结果表明TS对CS诱导的急性肺部炎症具有抗炎作用。TS治疗显着改善了CS暴露引起的气道上皮厚度，并导致肺趋化因子的产生显着减少。这些结果表明TS对CS诱导的急性肺部炎症具有抗炎作用。TS治疗显着改善了CS暴露引起的气道上皮厚度，并导致肺趋化因子的产生显着减少。这些结果表明TS对CS诱导的急性肺部炎症具有抗炎作用。