We developed a practical and reliable method for synthesizing an abasic deoxyribonucleoside, 1,2-dideoxy-d-ribofuranose (dR^H) via elimination of nucleobase from thymidine. To synthesize oligonucleotides bearing dR^H by the standard phosphoramidite solid-phase method, dR^H was converted to the corresponding phosphoramidite derivative and linked to a solid support (controlled pore glass resin). Chemically modified small interfering RNAs (siRNAs) possessing dR^H at their 3′-overhang regions were synthesized. Introducing dR^H to the 3′-end of the antisense strand of siRNA reduced its knockdown effect.

中文翻译：

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1,2-二脱氧-d-呋喃核糖的实用可靠合成方法及其在RNAi研究中的应用。

我们开发了用于合成无碱基脱氧核糖核苷，1,2-二脱氧一个实际和可靠的方法d -ribofuranose（DR ^ħ通过消除从胸苷碱基的）。为了通过标准亚磷酰胺固相法合成带有dR ^H的寡核苷酸，将dR ^H转化为相应的亚磷酰胺衍生物，并连接到固相载体（可控孔玻璃树脂）上。合成了在其3'-突出端区域具有dR ^H的化学修饰的小干扰RNA（siRNA）。将dR ^H引入siRNA反义链的3'末端可降低其敲低效应。