Pan_02鼠胰腺癌模型。,Folia medica Cracoviensia

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Pan_02鼠胰腺癌模型。
Folia medica Cracoviensia Pub Date : 2013-01-01
Marcin Przemysław Krzykawski ₁ , Martyna Krzykawska-Serda , Katarzyna Jasińska , Janusz Marcinkiewicz

Affiliation

目的本研究的目的是研究和描述Pan_02鼠胰腺胰腺癌肿瘤模型的基本特征。Pan_02对化学疗法的敏感性非常低，因此与人类胰腺癌非常相似。材料与方法给小鼠皮下注射不同数量的细胞，并测量肿瘤的生长。还使用多普勒模式的超声检查仪VEVO2100对肿瘤进行了研究，以检测存活和功能正常的血管。研究收集的肿瘤样品以评估坏死和血管通透性。结果我们发现，根据所接种肿瘤细胞的数量，肿瘤的生长存在显着差异。注射了0.5×106细胞的小鼠获得了最一致的生长方式。所有肿瘤均显示实质性血管形成，但较大的肿瘤更有可能形成较大的血管，而不是较密集的网络。结论鼠Pan_02癌症模型与人类PDAC癌症具有许多特征，因此它是研究新药的良好模型。注射0.5×106细胞可产生一致的结果，尽管它需要更多的时间才能使肿瘤生长。它还可以使免疫系统适应。由于具有良好的血管生成，Pan_02是研究针对胰腺腺癌的化学疗法的良好模型，但由于它对免疫刺激无反应，因此可能不是最佳的免疫疗法模型（未发表数据）。注射0.5×106细胞可产生一致的结果，尽管它需要更多的时间才能使肿瘤生长。它还可以使免疫系统适应。由于具有良好的血管生成，Pan_02是研究针对胰腺腺癌的化学疗法的良好模型，但由于它对免疫刺激无反应，因此可能不是最佳的免疫疗法模型（未发表数据）。注射0.5×106细胞可产生一致的结果，尽管它需要更多的时间才能使肿瘤生长。它还可以使免疫系统适应。由于具有良好的血管生成，Pan_02是研究针对胰腺腺癌的化学疗法的良好模型，但由于它对免疫刺激无反应，因此可能不是最佳的免疫疗法模型（未发表数据）。

"点击查看英文标题和摘要"

Pan_02 murine pancreatic cancer model.

AIM The aim of this study was to investigate and describe basic features of Pan_02 murine pancreatic ade- nocarcinoma tumor model. Pan_02 has very low sensitivity to chemotherapeutics therefore it is very similar to human pancreatic cancers. MATERIALS AND METHODS Mice were subcutaneously injected with different number of cells and tumor growth was measured. Tumors were also investigated with ultrasonograph VEVO2100 in doppler mode to detect viable and function- al blood vessels. Collected tumor samples were investigated to asses necrosis and blood vessel permeability. RESULTS We found substantial differences in tumor growth depending on a number of inoculated tumor cells. Mice injected with 0.5 × 106 cells gave the most consistent pattern of growth. All tumors showed substantial vascularisation but bigger tumors were more likely to develop larger blood vessels instead of a more dense network. CONCLUSIONS Murine Pan_02 cancer model shares many features with human PDAC cancers and therefore it is a good model to study new drugs. Injection of 0.5 × 106 cells gives consistent results although it requires more time for the tumor to grow. It also allows the immune system to adapt. Owing ta good vascularisation, Pan_02 is a good model to study chemotherapy against pancreatic adenocarcinoma but it may not be the best model for immunotherapy since it does not respond to the immune stimulation (unpublished data).

更新日期：2019-11-01

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