亚麻免疫受体L6和L7的比较分析提出了一种基于平衡的开关激活模型。,The Plant Cell

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亚麻免疫受体L6和L7的比较分析提出了一种基于平衡的开关激活模型。
The Plant Cell ( IF 10.0 ) Pub Date : 2016-01-09 , DOI: 10.1105/tpc.15.00303
Maud Bernoux ₁ , Hayden Burdett ₂ , Simon J Williams ₃ , Xiaoxiao Zhang ₄ , Chunhong Chen ₄ , Kim Newell ₄ , Gregory J Lawrence ₄ , Bostjan Kobe ₃ , Jeffrey G Ellis ₄ , Peter A Anderson ₂ , Peter N Dodds ₄

Affiliation

NOD样受体（NLR）是植物免疫系统的重要组成部分。L6是来自亚麻（Linum usitatissimum）的Toll /白介素1受体（TIR）域含NLR，可赋予对亚麻锈菌的免疫力。L6与弱等位基因L7的比较确定了TIR和核苷酸结合（NB）域中的两个多态性区域，该区域调节效应子配体依赖性和非依赖性细胞死亡信号传导以及核苷酸与受体的结合。这表明，TIR和NB域之间的负功能相互作用使L7处于非活性/ ADP结合状态比L6更紧密，因此降低了其采用活性/ ATP结合状态的能力，并解释了其在植物中的活性较弱。在酵母双杂交检测中，具有更稳定ADP结合状态的L6和L7变体未能与AvrL567结合，同时检测到与信号活性变体的结合。这与目前的模型相反，目前的模型预测效应子与非活性受体结合以触发激活。基于核苷酸结合，效应子相互作用和L6 / L7变体的免疫信号传导特性之间的相关性，我们提出NLRs存在于ON和OFF状态之间的平衡中，并且效应子与ON状态的结合稳定了这种构象，从而改变了平衡朝向受体的活性形式以触发防御信号。

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Comparative Analysis of the Flax Immune Receptors L6 and L7 Suggests an Equilibrium-Based Switch Activation Model.

NOD-like receptors (NLRs) are central components of the plant immune system. L6 is a Toll/interleukin-1 receptor (TIR) domain-containing NLR from flax (Linum usitatissimum) conferring immunity to the flax rust fungus. Comparison of L6 to the weaker allele L7 identified two polymorphic regions in the TIR and the nucleotide binding (NB) domains that regulate both effector ligand-dependent and -independent cell death signaling as well as nucleotide binding to the receptor. This suggests that a negative functional interaction between the TIR and NB domains holds L7 in an inactive/ADP-bound state more tightly than L6, hence decreasing its capacity to adopt the active/ATP-bound state and explaining its weaker activity in planta. L6 and L7 variants with a more stable ADP-bound state failed to bind to AvrL567 in yeast two-hybrid assays, while binding was detected to the signaling active variants. This contrasts with current models predicting that effectors bind to inactive receptors to trigger activation. Based on the correlation between nucleotide binding, effector interaction, and immune signaling properties of L6/L7 variants, we propose that NLRs exist in an equilibrium between ON and OFF states and that effector binding to the ON state stabilizes this conformation, thereby shifting the equilibrium toward the active form of the receptor to trigger defense signaling.

更新日期：2019-11-01

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