将糖苷水解酶大家族43划分为亚家族：详细表征酶的动机。,Applied and Environmental Microbiology

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将糖苷水解酶大家族43划分为亚家族：详细表征酶的动机。
Applied and Environmental Microbiology ( IF 3.9 ) Pub Date : 2016-01-06 , DOI: 10.1128/aem.03453-15
Keith Mewis ₁ , Nicolas Lenfant _{2,

3} , Vincent Lombard _{2,

3} , Bernard Henrissat _{3,

4,

5}

Affiliation

DNA测序的迅速增长导致糖苷水解酶（GH）家族数量的增加。GH43家族目前包含用于半纤维素和果胶聚合物脱支和降解的α-1-阿拉伯呋喃糖苷酶，β-d-木糖苷酶，α-1-阿拉伯糖苷酶和β-d-半乳糖苷酶。许多研究已经揭示了有关GH43成员的更详细的信息，这需要将GH43分为亚家族，就像以前对GH5和GH13家族所做的那样。此处介绍的工作是可靠的亚家族分类，可将91％的全部完整GH43结构域分配到37个亚家族中，这些亚家族与保守的序列残基以及生化测定和结构研究的结果相关。此外，这些亚科和其他功能模块的共现分析显示，某些GH43亚科与CBM6和CBM13结构域之间有很强的联系。共现分析还揭示了存在多达三个GH43结构域并属于不同亚家族的蛋白质，这表明每个亚家族的功能都有显着差异。总的来说，亚家族分析表明，GH43酶可能显示出迄今被低估的各种细微的特异性特征，当用简单的合成底物（例如pNP-糖苷）对这些酶进行分析时，这些特异性特征是不明显的。

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Dividing the Large Glycoside Hydrolase Family 43 into Subfamilies: a Motivation for Detailed Enzyme Characterization.

The rapid rise in DNA sequencing has led to an expansion in the number of glycoside hydrolase (GH) families. The GH43 family currently contains α-l-arabinofuranosidase, β-d-xylosidase, α-l-arabinanase, and β-d-galactosidase enzymes for the debranching and degradation of hemicellulose and pectin polymers. Many studies have revealed finer details about members of GH43 that necessitate the division of GH43 into subfamilies, as was done previously for the GH5 and GH13 families. The work presented here is a robust subfamily classification that assigns over 91% of all complete GH43 domains into 37 subfamilies that correlate with conserved sequence residues and results of biochemical assays and structural studies. Furthermore, cooccurrence analysis of these subfamilies and other functional modules revealed strong associations between some GH43 subfamilies and CBM6 and CBM13 domains. Cooccurrence analysis also revealed the presence of proteins containing up to three GH43 domains and belonging to different subfamilies, suggesting significant functional differences for each subfamily. Overall, the subfamily analysis suggests that the GH43 enzymes probably display a hitherto underestimated variety of subtle specificity features that are not apparent when the enzymes are assayed with simple synthetic substrates, such as pNP-glycosides.

更新日期：2019-11-01

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