不同来源的间充质干细胞：增殖能力，端粒长度和多能性标志物表达的比较评估。,Life Sciences

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不同来源的间充质干细胞：增殖能力，端粒长度和多能性标志物表达的比较评估。
Life Sciences ( IF 5.2 ) Pub Date : 2015-09-27 , DOI: 10.1016/j.lfs.2015.09.019
Drenka Trivanović ₁ , Aleksandra Jauković ₁ , Branka Popović ₂ , Jelena Krstić ₁ , Slavko Mojsilović ₁ , Ivana Okić-Djordjević ₁ , Tamara Kukolj ₁ , Hristina Obradović ₁ , Juan Francisco Santibanez ₁ , Diana Bugarski ₁

Affiliation

AIMS体外扩增改变了间充质干细胞（MSC）的复制和分化能力，增加了挑战和风险，同时限制了细胞疗法所需的足够数量的MSC。在这里，我们表征并比较了各种来源的MSC中的增殖，分化，端粒长度和多能性标记物表达。主要方法进行了免疫表型，增殖和分化测定。通过免疫荧光测定多能性标志物（Nanog，Oct-4，SOX-2，SSEA-4）的表达。进行定量PCR进行相对端粒长度（RTL）分析，同时表达多能性标记，分化状态（Cbfa1，人胎盘碱性磷酸酶，过氧化物酶体增殖物激活受体，Sox9和胶原IIa1）的相关基因，半定量RT-PCR检测端粒酶逆转录酶（hTERT）。主要发现外周血MSC（PB-MSC）和脐带MSC（UC-MSC）最高，而牙周膜MSC（PDL-MSC）和脂肪组织MSC（AT-MSC）的复制潜能值和最低值均最低。 RTL。尽管来自脱落乳牙（SHED），PDL-MSC和AT-MSC的MSC显示多能性标记的mRNA表达更高，但是所有MSC均表达多能性标记蛋白。SHEDs和PDL-MSCs具有显着的成骨能力，PB-MSCs和UC-MSCs具有增强的成脂分化潜能，而AT-MSCs表现出相似的分化能力。意义尽管显示相似的表型，但来自不同来源的MSCs群体，在与其自我更新和分化能力有关的生物学特性方面表现出高度的可变性。这些数据表明，为了更准确地用于细胞治疗，应确定从不同组织分离的MSC的个性，并在计划将其用于临床方案时予以考虑。

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Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression.

AIMS In vitro expansion changes replication and differentiation capacity of mesenchymal stem cells (MSCs), increasing challenges and risks, while limiting the sufficient number of MSCs required for cytotherapy. Here, we characterized and compared proliferation, differentiation, telomere length and pluripotency marker expression in MSCs of various origins. MAIN METHODS Immunophenotyping, proliferation and differentiation assays were performed. Pluripotency marker (Nanog, Oct-4, SOX-2, SSEA-4) expression was determined by immunofluorescence. Quantitative PCR was performed for relative telomere length (RTL) analyses, while expression of relevant genes for pluripotency markers, differentiation state (Cbfa1, human placental alkaline phosphatase, peroxisome proliferator activated receptor, Sox9 and Collagen II a1), and telomerase reverse transcriptase (hTERT) was determined by semiquantitative RT-PCR. KEY FINDINGS Peripheral blood MSCs (PB-MSCs) and umbilical cord MSCs (UC-MSCs) showed the highest, while periodontal ligament MSCs (PDL-MSCs) and adipose tissue MSCs (AT-MSCs) the lowest values of both the replication potential and RTL. Although MSCs from exfoliated deciduous teeth (SHEDs), PDL-MSCs and AT-MSCs showed higher mRNA expression of pluripotency markers, all MSCs expressed pluripotency marker proteins. SHEDs and PDL-MSCs showed prominent capacity for osteogenesis, PB-MSCs and UC-MSCs showed strengthened adipogenic differentiation potential, while AT-MSCs displayed similar differentiation into both lines. SIGNIFICANCE The MSCs populations derived from different sources, although displaying similar phenotype, exhibited high degree of variability regarding biological properties related to their self-renewal and differentiation capacity. These data indicate that for more accurate use in cell therapy, individualities of MSCs isolated from different tissues should be identified and taken into consideration when planning their use in clinical protocols.

更新日期：2015-09-25

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