马齿ula多糖对小鼠骨髓树突状细胞表型和功能成熟的影响。,Nutrition and Cancer

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马齿ula多糖对小鼠骨髓树突状细胞表型和功能成熟的影响。
Nutrition and Cancer ( IF 2.0 ) Pub Date : 2015-07-30 , DOI: 10.1080/01635581.2015.1060352
Rui Zhao ₁ , Tao Zhang , Hui Zhao , Yaping Cai

Affiliation

Portulaca oleracea L.是一年生植物，广泛分布于温带至热带地区。POL-P3b是从马齿Port（Portulaca oleracea L.）纯化的多糖级分，能够增强免疫力并抑制肿瘤形成。树突状肿瘤融合细胞诱导的抗肿瘤免疫可以通过其激活状态来调节。成熟的树突状细胞在细胞毒性T淋巴细胞诱导方面明显优于未成熟的树突状细胞。在这项研究中，我们分析了POL-P3b对小鼠骨髓源性树突状细胞（DCs）的成熟和功能的影响及其相关机制。通过流式细胞术证实DC的表型成熟。我们发现POL-P3b上调了DC上CD80，CD86，CD83和主要的组织相容性复合物II类分子的表达，刺激产生更多的白介素（IL）-12，肿瘤坏死因子-α和更少的IL-10。另外，DCs脉冲POL-P3b和冻融抗原可增加DCs驱动的T细胞的增殖并促进U14细胞的凋亡。此外，TLR-4的表达在POL-P3b处理的DC上显着增加。这些结果表明，POL-P3b可能通过TLR-4诱导DC成熟。综上所述，我们的研究结果可能对POL-P3b免疫增强的分子机制具有重要意义，并提供直接证据表明POL-P3b应该被认为是基于DC的疫苗的有效佐剂营养补充剂。TLR-4的表达在POL-P3b处理的DC上显着增加。这些结果表明，POL-P3b可能通过TLR-4诱导DC成熟。综上所述，我们的研究结果可能对POL-P3b免疫增强的分子机制具有重要意义，并提供直接证据表明POL-P3b应该被认为是基于DC的疫苗的有效佐剂营养补充剂。TLR-4的表达在POL-P3b处理的DC上显着增加。这些结果表明，POL-P3b可能通过TLR-4诱导DC成熟。综上所述，我们的研究结果可能对POL-P3b免疫增强的分子机制具有重要意义，并提供直接证据表明POL-P3b应该被认为是基于DC的疫苗的有效佐剂营养补充剂。

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Effects of Portulaca oleracea L. Polysaccharides on Phenotypic and Functional Maturation of Murine Bone Marrow Derived Dendritic Cells.

Portulaca oleracea L. is an annual plant widely distributed from the temperate to the tropical zones. POL-P3b, a polysaccharide fraction purified from Portulaca oleracea L., is able to enhance immunity and inhibit tumor formation. Induction of antitumor immunity by dendritic-tumor fusion cells can be modulated by their activation status. Mature dendritic cells are significantly better than immature dendritic cells at cytotoxic T-lymphocyte induction. In this study, we analyzed the effects of POL-P3b on the maturation and function of murine bone-marrow-derived dendritic cells (DCs) and relevant mechanisms. The phenotypic maturation of DCs was confirmed by flow cytometry. We found that POL-P3b upregulated the expression of CD80, CD86, CD83, and major histocompatibility complex class II molecules on DCs, stimulated production of more interleukin (IL)-12, tumor necrosis factor-α, and less IL-10. Also, DCs pulsed POL-P3b and freeze-thaw antigen increased DCs-driven T cells' proliferation and promoted U14 cells' apoptosis. Furthermore, the expression of TLR-4 was significantly increased on DCs treated by POL-P3b. These results suggested that POL-P3b may induce DCs maturation through TLR-4. Taken together, our results may have important implications for the molecular mechanisms of immunopotentiation of POL-P3b, and provide direct evidence to suggest that POL-P3b should be considered as a potent adjuvant nutrient supplement for DC-based vaccines.

更新日期：2019-11-01

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