植物病原性卵菌疫霉（Phytophthora capsici）对杀真菌剂pyrmorph的响应。,Proteomics

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植物病原性卵菌疫霉（Phytophthora capsici）对杀真菌剂pyrmorph的响应。
Proteomics ( IF 3.4 ) Pub Date : 2015-04-29 , DOI: 10.1002/pmic.201400502
Zhili Pang _{1,

2} , Lei Chen _{1,

3} , Jianqiang Miao ₁ , Zhiwen Wang ₁ , Vincent Bulone _{2,

4} , Xili Liu ₁

Affiliation

Pyrimorph是来自羧酸酰胺（CAA）家族的新型杀菌剂，用于控制植物致病卵菌，如辣椒疫霉菌。使用iTRAQ技术研究了辣椒疫霉菌对吡虫啉的蛋白质组学响应，以确定杀真菌剂的目标位点和潜在的候选药物疗效生物标志物。从野生型辣椒毕赤酵母分离物（Hd3）的菌丝体中鉴定出总共1336种独特蛋白质，并在存在或不存在pyrmorph的情况下生长出两个耐pyrmorph的突变体（R3-1和R3-2）。比较分析表明，三个辣椒辣椒分离株Hd3，R3-1和R3-2分别产生163、77和13个独特的蛋白质，这些蛋白质表现出响应于硫铁矿处理的丰度变化。进一步调查，使用直系同源蛋白质组（COG）分析和《京都议定书》的基因和基因组百科全书（KEGG）途径分析，鉴定了35种与pyrmorph对抗辣椒疫霉的作用方式有关的蛋白质和62种参与辣椒疫霉胁迫响应的蛋白质到pyrmorph。许多表达改变的蛋白质与葡萄糖和能量代谢有关。使用d- [U-（14）C]葡萄糖的生化分析验证了蛋白质组学数据，表明辣椒辣椒中pyrmorph的主要作用方式是抑制细胞壁的生物合成。这些结果还表明，蛋白质组学方法是确定新型杀真菌剂靶向途径以及评估植物病原体对环境挑战（例如杀真菌剂的存在）的耐受性的有用工具。

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Proteomic profile of the plant-pathogenic oomycete Phytophthora capsici in response to the fungicide pyrimorph.

Pyrimorph is a novel fungicide from the carboxylic acid amide (CAA) family used to control plant-pathogenic oomycetes such as Phytophthora capsici. The proteomic response of P. capsici to pyrimorph was investigated using the iTRAQ technology to determine the target site of the fungicide and potential biomarker candidates of drug efficacy. A total of 1336 unique proteins were identified from the mycelium of wild-type P. capsici isolate (Hd3) and two pyrimorph-resistant mutants (R3-1 and R3-2) grown in the presence or absence of pyrimorph. Comparative analysis revealed that the three P. capsici isolates Hd3, R3-1, and R3-2 produced 163, 77, and 13 unique proteins, respectively, which exhibited altered levels of abundance in response to the pyrimorph treatment. Further investigations, using Cluster of Orthologous Groups of Proteins (COG) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis identified 35 proteins related to the mode of action of pyrimorph against P. capsici and 62 proteins involved in the stress response of P. capsici to pyrimorph. Many of the proteins with altered expression were associated with glucose and energy metabolism. Biochemical analysis using d-[U-(14) C]glucose verified the proteomics data, suggesting that the major mode of action of pyrimorph in P. capsici is the inhibition of cell wall biosynthesis. These results also illustrate that proteomics approaches are useful tools for determining the pathways targeted by novel fungicides as well as for evaluating the tolerance of plant pathogens to environmental challenges, such as the presence of fungicides.

更新日期：2015-06-08

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